Rat mast cell phospholipase A2: activity toward exogenous phosphatidylserine and inhibiton by N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylserine.
Rat mast cell phospholipase A2: activity toward exogenous phosphatidylserine and inhibiton by N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylserine.
复制标题
大鼠肥大细胞磷脂酶 A2：对外源磷脂酰丝氨酸的活性和 N-(7-硝基-2,1,3-苯并恶二唑-4-基)磷脂酰丝氨酸的抑制。
DOI：
10.1021/bi00535a023
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Lagunoff,D
中科院分区：
文献类型：
--
作者：
Martin,TW;Lagunoff,D
Thomas W. Martin and David Lagunoff* abstract: The presence of phospholipase A2 in intact rat peritoneal mast cells was investigated by using two synthetic radiolabeled phosphatidylserine (PS) substrates. Incubation of intact cells with 1-oleoyl-2-[3H] oleoyl-PS resulted in the release of a considerable quantity of [3H] oleic acid from the substrate. To establish that [3H] oleic acid release was me-diated via direct enzymatic attack at the sn-2 position, we measured release of the [3H] serine moiety from the glycerol backbone of 1, 2-dimyristoylphosphatidyl [3H] serine. This activity, which represents the combined actions of phospho-lipases C and D, was 10-fold lower than [3H] oleic acid release, indicating that neither of these enzymes is required for the release of the preponderance of [3H] oleic acid. These results establish the existence in intact rat mast cells of a phospho-
