An Essential Factor for High Mg(2+) Tolerance of Staphylococcus aureus.
An Essential Factor for High Mg(2+) Tolerance of Staphylococcus aureus.
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DOI：
10.3389/fmicb.2016.01888
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发表时间：
2016
影响因子：
5.2
通讯作者：
Linder P
中科院分区：
文献类型：
--
作者：
Armitano J;Redder P;Guimarães VA;Linder P
活细胞中最丰富的二价阳离子Mg 2+的内部细菌浓度估计在单毫摩尔范围内。然而，许多细菌将通过使用一系列深入研究和高效的输入机制在仅具有微摩尔的Mg2+的培养基中茁壮成长，以及在具有非常高的镁浓度的培养基中茁壮成长，推测由目前未知的输出机制介导。金黄色葡萄球菌对病原体具有特别高的Mg2+耐受性，在高达770 mM的Mg2+中生长不受损害，我们在这里鉴定了SA0657，这是这种耐受性的关键因素。SA0657的预测结构域结构与细菌、古细菌甚至真核生物中的大量蛋白质共享，例如来自沙门氏菌的CorB和人CNNM蛋白质家族。其中一个共享的结构域，CBS对可能参与Mg 2+传感，包含保守的甘氨酸326，我们建立了SA0657功能的关键残基。根据我们的发现，我们建议将SA0657命名为MpfA，即镁保护因子A。
Internal bacterial concentration of Mg2+, the most abundant divalent cation in living cells, is estimated to be in the single millimolar range. However, many bacteria will thrive in media with only micromolars of Mg2+, by using a range of intensely studied and highly efficient import mechanisms, as well as in media with very high magnesium concentration, presumably mediated by currently unknown export mechanisms. Staphylococcus aureus has a particularly high Mg2+ tolerance for a pathogen, growing unimpaired in up to 770 mM Mg2+, and we here identify SA0657, a key factor in this tolerance. The predicted domain structure of SA0657 is shared with a large number of proteins in bacteria, archaea and even eukarya, for example CorB from Salmonella and the human CNNM protein family. One of the shared domains, a CBS pair potentially involved in Mg2+ sensing, contains the conserved Glycine326 which we establish to be a key residue for SA0657 function. In light of our findings, we propose the name MpfA, Magnesium Protection Factor A, for SA0657.