Carcinoma cell-specific Mig-7: a new potential marker for circulating and migrating cancer cells.
Carcinoma cell-specific Mig-7: a new potential marker for circulating and migrating cancer cells.
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DOI：
10.3892/or.13.1.37
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发表时间：
2005
期刊：
影响因子：
4.2
通讯作者：
T. Phillips;J. Lindsey
中科院分区：
文献类型：
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作者：
T. Phillips;J. Lindsey
以癌细胞特异性方式表达的基因的鉴定可以提供用于检测、诊断和疾病进展的标志物。我们以前曾报道，受体酪氨酸激酶配体与α v β 5整合素的连接诱导Mig-7的表达仅限于癌细胞。由于这种高度特异性的表达，我们假设Mig-7可以用作隐匿性肿瘤细胞的标志物。本研究的目的是开始通过制备Mig-7特异性抗血清和RT-PCR方法检测Mig-7在癌症患者组织和血液中的表达，并与正常受试者的组织和血液进行比较，来验证这一假设。通过免疫组织化学和RT-PCR，我们在9只子宫内膜癌异种移植小鼠中的7只（77.8%）的淋巴结中检测到Mig-7 mRNA，而在5只阴性对照动物中没有检测到。与正常子宫内膜组织样品相比，子宫内膜癌中Mig-7表达比Met表达更具特异性，Met表达是结合散射因子的RTK，并用作进展不良的标志物。在87.3%的乳腺、肺、结肠和卵巢肿瘤中，我们检测到Mig-7的表达。未经治疗的转移性癌症患者的血液样本也显示出Mig-7 mRNA，而接受化疗的患者或正常人则缺乏表达。总之，我们报告的第一个免疫组化和RT-PCR检测米格-7和讨论其高度特异性定位于癌细胞中的情况下，在正常细胞。我们的初步数据表明，Mig-7可能是一个潜在的早期标记迁移和循环癌细胞。
Identification of genes that are expressed in a cancer cell-specific manner can provide markers for detection, diagnosis, and disease progression. We have previously reported that receptor tyrosine kinase ligands in concert with ligation of alphavbeta5 integrin induce expression of Mig-7 restricted to carcinoma cells. Because of this highly specific expression, we hypothesized that Mig-7 could be used as a marker of occult tumor cells. The objective of this study was to begin to test this hypothesis by generating Mig-7 specific antisera and RT-PCR methods for detection of Mig-7 expression in tissues and blood from cancer patients as compared to those from normal subjects. By immunohistochemistry and by RT-PCR, we detected Mig-7 mRNA in lymph nodes from 7 out of 9 (77.8%) endometrial carcinoma xenograft mice but not from any of the 5 negative control animals. Mig-7 expression was more specific than Met expression, the RTK that binds Scatter factor and is used as a marker of poor progression, in endometrial carcinoma as compared to normal endometrial tissue samples. In 87.3% of tumors from various tissues including breast, lung, colon and ovary, we detected Mig-7 expression. Blood samples from untreated metastatic cancer patients also displayed Mig-7 mRNA in contrast to a lack of expression in chemotherapy treated or normal individuals. In conclusion, we report the first immunohistochemical and RT-PCR assays for Mig-7 and discuss its highly specific localization to cancer cells in contrast to an absence in normal cells. Our preliminary data indicate that Mig-7 may be a potential early marker of migrating and circulating carcinoma cells.