Development of photo-controllable hydrogen sulfide donor applicable in live cells
Development of photo-controllable hydrogen sulfide donor applicable in live cells
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DOI：
10.1016/j.bmcl.2014.11.084
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发表时间：
2015-01-15
影响因子：
2.7
通讯作者：
Nakagawa, Hidehiko
中科院分区：
文献类型：
--
作者：
Fukushima, Naoki;Ieda, Naoya;Nakagawa, Hidehiko
硫化氢（H2S）具有多种生理作用，例如在血管舒张和炎症中。在环境条件下，它是一种高度反应性的气体，因此需要可控的H2S供体来研究其生物功能。在这里，我们描述了H2S供体（SPD-2），利用氧杂蒽酮光化学控制H2S释放的设计，合成和应用。SPD-2产生的H2S完全依赖于UVA照射（325-385 nm），如通过亚甲蓝测定和使用H2S选择性荧光探针所证实的。SPD-2被证实在活细胞中提供受控的H2S递送，并且应该适合于各种生物应用。(C)2014作者爱思唯尔有限公司出版
Hydrogen sulfide (H2S) has multiple physiological roles, for example, in vasodilation and inflammation. It is a highly reactive gas under ambient conditions, so controllable H2S donors are required for studying its biological functions. Here, we describe the design, synthesis and application of a H2S donor (SPD-2) that utilizes xanthone photochemistry to control H2S release. H2S generation from SPD-2 was completely dependent on UVA-irradiation (325-385 nm), as confirmed by methylene blue assay and by the use of a H2S-selective fluorescent probe. SPD-2 was confirmed to provide controlled H2S delivery in live cells, and should be suitable for various biological applications. (C) 2014 The Authors. Published by Elsevier Ltd.