Relationship between a toll-like receptor-4 gene polymorphism, bacterial vaginosis-related flora and vaginal cytokine responses in pregnant women
Relationship between a toll-like receptor-4 gene polymorphism, bacterial vaginosis-related flora and vaginal cytokine responses in pregnant women
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DOI：
10.1016/j.ejogrb.2004.02.010
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发表时间：
2004-10-15
影响因子：
2.6
通讯作者：
Witkin, SS
中科院分区：
文献类型：
--
作者：
Genc, MR;Vardhana, S;Witkin, SS
目的：对孕妇Toll样受体4（TLR 4）基因单核苷酸多态性（TLR 4 896 A > G）、阴道菌群的定性和定量变化与阴道白细胞介素（IL）-1 β和IL-1受体拮抗剂（IL-1 ra）浓度之间的关系进行了评价。研究设计：采用定性和定量微生物方法对238例妊娠18-22周的妇女进行阴道微生物区系分析。采用聚合酶链反应检测TLR 4基因型。通过ELISA测定阴道灌洗液样品中的IL-1 β和IL 1 ra浓度。结果：在10.3%的女性中发现了TLR 4变异体。携带这种变异体与阴道pH值的中位数增加（P = 0.05）、阴道加德纳菌水平增加10倍以上（P < 0.0001）以及三种厌氧革兰氏阴性杆菌（普雷沃氏菌、拟杆菌和卟啉单胞菌）的阴道浓度增加10倍（P = 0.08）相关。定殖G.在TLR 4 896 A纯合子的妇女中，阴道分泌物和/或厌氧性革兰氏阴性杆菌导致阴道IL-1（P = 0.01）和IL-1 ra（P < 0.0002）浓度升高，但在TLR 4 896 G携带者中没有。结论：TLR 4896 A > G多态性是导致阴道抗G抗体个体差异的重要原因。迷走神经和厌氧革兰氏阴性杆菌。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Objective: The relationship between a single nucleotide polymorphism (TLR4 896 A > G) in the toll-like receptor-4 (TLR4) gene, qualitative and quantitative changes in vaginal micro-flora and vaginal interleukin (IL)-1beta and IL-1 receptor antagonist (IL-1ra) concentrations in pregnant women were evaluated. Study design: Qualitative and quantitative microbial methods were used to characterize vaginal micro-flora of 238 women at 18-22 weeks gestation. Polymerase chain reaction was used to determine TLR4 genotype. IL-1beta and IL1ra concentrations in vaginal lavage samples were measured by ELISA. Results: The TLR4 variant was identified in 10.3% of women. Carriage of this variant was associated with a median increase in vaginal pH (P = 0.05), a greater than 10-fold increase in vaginal Gardnerella vaginalis levels (P < 0.0001) and a 10-fold increase in the vaginal concentration of three species of anaerobic Gram-negative rods, Prevotella, Bacteroides, and Porphyromonas (P = 0.08). Colonization with G. vaginalis and/or the anaerobic Gram-negative rods resulted in elevated vaginal IL-1 (P = 0.01) and IL-1ra (P < 0.0002) concentrations in women who were TLR4 896A homozygotes, but not in TLR4 896G carriers. Conclusion: The TLR4 896 A > G polymorphism contributes to inter-individual differences in the vaginal immune defense against G. vaginalis and anaerobic Gram-negative rods. (C) 2004 Elsevier Ireland Ltd. All rights reserved.