Endogenous galanin modulates the gonadotropin and prolactin proestrous surges in the rat.
Endogenous galanin modulates the gonadotropin and prolactin proestrous surges in the rat.
复制标题
内源性甘丙肽调节大鼠发情前的促性腺激素和催乳素激增。
作者：
F. J. López;E. H. Meade;A. Negro
甘丙氨酸（galanine， GAL）作为一种重要的神经内分泌调节剂，参与控制垂体和下丘脑的多种激素。我们之前观察到GAL刺激正中隆起神经末梢的LHRH释放，以及体外垂体细胞的基础LH和LHRH诱导的LH分泌，这促使我们评估内源性GAL是否在调节生理性发生的促性腺激素和PRL的排卵前激增中起作用。采用高亲和羊抗大鼠GAL血清（FMS-FJL 17-5）对发情雌性大鼠进行GAL被动免疫。动物在心衰期间植入留置心房导管。在预期的发情日，大鼠在开始采血前1小时分别注射1 ml正常绵羊血清或GAL抗血清（GAL- as）。从1400-2300小时每小时采集0.5 ml血样，用RIA测定血浆LH、FSH和PRL水平。在GAL-AS给药后的几个时间间隔内，使用标准饱和度测定法评估大鼠血浆的最大结合能力。在整个实验期间存在高中和水平的免疫球蛋白。GAL被动免疫可使LH排卵前激增30%。虽然最大LH水平不受治疗影响，但在1700、1900和2000 h时，分泌曲线下面积和LH水平显著降低。相反，FSH分泌在最大FSH水平或曲线下面积方面没有显著改变。然而，GAL- as处理的大鼠在1700 h时FSH水平显著降低。GAL被动免疫选择性地减少了PRL排卵前激增的平台期。在峰值起始或最高PRL水平上没有观察到显著差异，而PRL水平在1700至2200小时显著降低。此外，gal - as处理的动物PRL曲线下的面积减少了40%。总之，我们的研究结果清楚地表明，内源性GAL参与控制排卵前LH和PRL的激增，而不改变FSH激增。此外，他们首次提供了内源性GAL在调节导致排卵的生理事件中的重要作用的证据。
Galanin (GAL) has recently emerged as an important neuroendocrine regulator which participates in the control of several pituitary and hypothalamic hormones. Our earlier observation that GAL stimulates LHRH release from nerve terminals of the median eminence as well as basal LH and LHRH-induced LH secretion from pituitary cells in vitro prompted us to evaluate whether endogenous GAL plays a role in regulation of the physiologically occurring preovulatory surges of gonadotropins and PRL. Proestrous female rats were passively immunized against GAL using a high affinity sheep antirat GAL serum (FMS-FJL 17-5). Animals were implanted during diestrus with indwelling atrial cannulae. On the expected day of proestrus, rats received 1 ml of either normal sheep serum or GAL antiserum (GAL-AS), iv, 1 h before blood sampling started. Blood samples (0.5 ml) were collected at hourly intervals from 1400-2300 h, and plasma levels of LH, FSH, and PRL measured by RIA. At several time intervals after GAL-AS administration, the maximum binding ability of the rat plasma was evaluated using standard saturation assays. High neutralizing levels of immunoglobulins were present throughout the experimental period. GAL passive immunization blunted the LH preovulatory surge by 30%. Although maximum LH levels were unaffected by the treatment, the area under the secretory curve and LH levels at 1700, 1900, and 2000 h were significantly reduced. Conversely, FSH secretion was not significantly altered for either maximum FSH levels or area under the curve. However, FSH levels were significantly diminished in GAL-AS-treated rats at 1700 h. GAL passive immunization selectively reduced the plateau phase of the preovulatory surge of PRL. No significant differences were observed in the initiation of the surge or maximum PRL levels, whereas PRL levels were significantly reduced from 1700 to 2200 h. In addition, the area under the PRL curve was diminished in GAL-AS-treated animals by 40%. In conclusion, our results clearly demonstrate that endogenous GAL is involved in control of the preovulatory surges of LH and PRL without altering the FSH surge. In addition, they provide, for the first time, evidence of an important role for endogenous GAL in the regulation of physiological events leading to ovulation.
影响因子：
4.8
作者：
L. Arbogast;N. Ben-Jonathan
通讯作者：
L. Arbogast;N. Ben-Jonathan
甘丙肽在控制下丘脑垂体前叶功能中的调节作用。
DOI：
10.1073/pnas.85.24.9861
发表时间：
1988
影响因子：
11.1
作者：
Ottlecz,A;Snyder,GD;McCann,SM
通讯作者：
McCann,SM