Similarities in the light chains of anti-gamma-globulins showing cross-idiotypic specificities.
Similarities in the light chains of anti-gamma-globulins showing cross-idiotypic specificities.
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DOI：
10.1084/jem.139.1.128
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
Capra JD
中科院分区：
文献类型：
--
作者：
Kunkel HG;Winchester RJ;Joslin FG;Capra JD
在对17种具有抗-γ-球蛋白活性的IgM蛋白的分析中观察到轻链的显著均一性。轻链的V区亚组通过序列和抗原分析确定。后一种方法允许大规模筛选以与对照蛋白进行比较。这两种方法在Kappa III蛋白的测定中表现出普遍的一致性;所有抗原分析阳性的蛋白质序列也是阳性的，但在相反的方向上有例外。通过抗原分析，抗γ球蛋白显示VK III轻链的发生率为92%，而81种无此活性的对照蛋白的发生率为27%。观察到一个类似的选择抗原（VK III B）细分的κ III蛋白。抗γ-球蛋白的主要Wa组（先前已根据交叉独特型特异性进行了描述）主要负责特殊轻链选择。该组的所有蛋白质均含有VK III轻链，并且均为VK III B型。目前的证据表明，额外的轻链相似性涉及跨独特型特异性的Wa组。因此，似乎对于抗γ球蛋白，轻链的不同选择水平是明显的，从κ型、κ III亚组、κ III B亚组的优势到可能的进一步亚组的优势，以解释交叉独特型特异性。
A marked homogeneity of the light chains was observed in an analysis of 17 IgM proteins with anti-γ-globulin activity. The V region subgroups of the light chains were determined by both sequence and antigenic analysis. The latter procedure permitted large scale screening for comparisons with control proteins. The two methods showed general agreement in the determination of Kappa III proteins; all proteins positive by antigenic analysis were also positive by sequence but exceptions were noted in the opposite direction. The anti-γ-globulins showed by antigenic analysis a 92% incidence of VK III light chains as compared to an incidence of 27% among 81 control proteins without this activity. A similar selection was observed for an antigen (VK III b) which subdivided the kappa III proteins. The major Wa group of anti-γ-globulins which had been delineated previously on the basis of cross-idiotypic specificity was primarily responsible for the special light-chain selection. All the proteins of this group contain VK III light chains and all were of the VK III b type. Current evidence indicates that additional light-chain similarities were involved in the cross-idiotypic specificity of the Wa group. It thus appears that for the anti-γ-globulins various levels of selection of light chains are manifest ranging from a preponderance of kappa type, of kappa III subgroup, of kappa III b sub-subgroup and perhaps of still further subdivisions to account for the cross-idiotypic specificity.