Generation of highly homogeneous strains of zebrafish through full sib-pair mating.
Generation of highly homogeneous strains of zebrafish through full sib-pair mating.
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DOI：
10.1534/g3.111.000851
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Sakai N
中科院分区：
文献类型：
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作者：
Shinya M;Sakai N
遗传同质群体，如近交系，是强大的实验工具，非常适合研究免疫学，癌症和复杂性状的遗传学。斑马鱼，Danio rerio，在这些研究领域一直未得到充分利用，因为实验鱼的同质品系还没有在驯服的条件下。在这里，我们试图近亲繁殖两个斑马鱼野生型菌株，蒂宾根和印度，通过完整的同胞对交配。虽然近交系图宾根株未能茁壮成长，并在13代后丢失，但近交系印度株（IM）已成功维持。IM品系经历了16代近亲繁殖，并保持了健康的状态。另外两个菌株IM 12 m和IM 14 m建立为来自IM菌株分支的封闭菌落。使用遗传标记的基因型分析显示，在IM（第14代）和两个封闭的殖民地的多态性显着减少（62%下降到5%）。这表明这些菌株具有高度的同质性。此外，每个品系内的个体之间的规模移植是成功的。这些数据表明，已经建立了非常同质的斑马鱼品系，从而为实际应用创造了宝贵的资源。
Genetically homogeneous populations, such as inbred strains, are powerful experimental tools that are ideally suited for studying immunology, cancer, and genetics of complex traits. The zebrafish, Danio rerio, has been underutilized in these research areas because homogeneous strains of experimental fish have not been available in tractable condition. Here, we attempted to inbreed two zebrafish wild-type strains, Tübingen and India, through full sib-pair mating. Although the inbred Tübingen strain failed to thrive and was lost after 13 generations, an inbred India strain (IM) has been maintained successfully. The IM strain has endured 16 generations of inbreeding and has maintained a healthy condition. Two additional strains, IM12m and IM14m, were established as closed colonies from the branches of the IM strain. Genotype analyses using genetic markers revealed a dramatic decrease in polymorphisms (62% dropped to 5%) in both IM (generation 14) and the two closed colonies. This indicates a high level of homogeneity in these strains. Furthermore, scale transplantations between individuals within each strain were successful. These data suggest that extremely homogeneous zebrafish strains have been established, thereby creating a valuable resource for practical application.