Effect of dexamethasone on extracellular secretion of cystatin C in cancer cell lines.
Effect of dexamethasone on extracellular secretion of cystatin C in cancer cell lines.
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DOI：
10.3892/br.2012.21
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发表时间：
2013
影响因子：
2.3
通讯作者：
C. Yamawaki;Minoru Takahashi;K. Takara;M. Kume;M. Hirai;H. Yasui;Tsutomu Nakamura
中科院分区：
文献类型：
--
作者：
C. Yamawaki;Minoru Takahashi;K. Takara;M. Kume;M. Hirai;H. Yasui;Tsutomu Nakamura
本研究旨在探讨地塞米松（DEX）诱导的人癌细胞Cys C分泌及顺铂（CDDP）和5-氟尿嘧啶（5-FU）对Cys C分泌的影响。将KYSE 150、A549和Caki-2人癌细胞系在塑料皿上培养并用DEX（100 nM）处理24、48和72小时。KYSE 150细胞用DEX、CDDP（10 μ M）和5-FU（2 μ M）共处理。评价DEX、CDDP和5-FU对细胞活力的影响。结果显示，DEX处理的KYSE 150细胞的培养基中的Cys C分泌水平比对照细胞的培养基中的Cys C分泌水平高1.8至2.3倍。在所有时间点在A549细胞中观察到类似的趋势，而仅在DEX处理后24 h观察到Caki-2细胞的Cys C分泌显著增加。对于KYSE 150细胞，Cys C的分泌也通过CDDP或5-FU与DEX的共处理而增强，尽管它不受DEX和米非司酮（糖皮质激素受体拮抗剂）的共施用的影响。在食管癌化疗中通常使用的浓度下，与DEX相比，CDDP和5-FU在KYSE 150细胞中表现出中等水平的细胞毒性。这些结果表明，DEX具有增强食管癌细胞Cys C的胞外分泌的潜力，可能是由于糖皮质激素受体活性介导的转录调节。
The aim of the present study was to investigate dexamethasone (DEX)-induced secretion of cystatin C (Cys C) and the effect of cisplatin (CDDP) and 5-fluorouracil (5-FU) on Cys C secretion in human cancer cell lines. KYSE150, A549 and Caki-2 human cancer cell lines were cultured on plastic dishes and treated with DEX (100 nM) for 24, 48 and 72 h. KYSE150 cells were co-treated with DEX, CDDP (10 μM), and 5-FU (2 μM). The effects of DEX, CDDP and 5-FU on cell viability were evaluated. Results showed Cys C secretion levels in the culture medium of DEX-treated KYSE150 cells to be 1.8- to 2.3-fold higher compared to those in the culture medium of control cells. A similar tendency was observed in A549 cells at all the time points, whereas a significant increase in the Cys C secretion by Caki-2 cells was observed only 24 h after DEX treatment. Regarding KYSE150 cells, the secretion of Cys C was also enhanced by co-treatment of CDDP or 5-FU with DEX, although it was not affected by the co-administration of DEX and mifepristone, a glucocorticoid receptor antagonist. At concentrations that are typically used in esophageal cancer chemotherapy, CDDP and 5-FU demonstrated a moderate level of cytotoxicity in KYSE150 cells in contrast to DEX. These findings suggested that DEX has the potential to enhance the extracellular secretion of Cys C in esophageal cancer cells, possibly due to the transcriptional regulation mediated by glucocorticoid receptor activity.