Expression of stress proteins (HSP-70 and HSP-90) in the rabbit urinary bladder subjected to partial outlet obstruction.
Expression of stress proteins (HSP-70 and HSP-90) in the rabbit urinary bladder subjected to partial outlet obstruction.
复制标题
部分出口梗阻的兔膀胱中应激蛋白（HSP-70 和 HSP-90）的表达。
DOI：
10.1007/bf01084267
复制
发表时间：
1994
影响因子：
4.3
通讯作者：
Levin,RM
中科院分区：
文献类型：
--
作者：
Zhao,Y;Chacko,S;Levin,RM
兔膀胱出口部分梗阻引起快速肥大，表现为膀胱质量增加、平滑肌含量增加和胶原沉积增加。此外，部分出口梗阻导致对野刺激和突触后受体刺激的收缩反应减弱。虽然对部分出口阻塞的形态学和收缩反应已经很好地表征，但这些变化的细胞和分子机制的信息很少。在之前的研究中，我们证实了兔部分出口梗阻后最早表达的基因之一是表达应激蛋白70 （HSP-70）的基因。为了进一步明确这些反应的遗传和分子基础，我们使用抗HSP-70和90单克隆抗体，采用Western blot联合激光密度测定法定量评价应激基因产物HSP-70和HSP-90在部分出口梗阻兔膀胱中的表达。结果表明，应激基因产物HSP-70和HSP-90在对照兔膀胱组织中组成性表达，部分出口受阻后短暂性表达增加。在梗阻后6小时检测到HSP-70的含量增加，并在24小时达到最大值（是对照水平的2.7倍）。6小时时也检测到HSP-90升高，但在12小时时达到最大值（比对照水平高4.5倍）。梗阻后7 d，这两种蛋白的含量恢复到对照水平。本研究提示应激基因表达的改变导致HSP-70和90的升高可能在膀胱对部分出口梗阻的反应中起重要作用。
Partial obstruction of the rabbit bladder outlet induces a rapid hypertrophy characterized by increased bladder mass, increased smooth muscle content, and increased collagen deposition. In addition, partial outlet obstruction induces decreased contractile responses to both field stimulation and postsynaptic receptor stimulation. Although the morphological and contractile responses to partial outlet obstruction have been well characterized, there is little information on the cellular and molecular mechanisms of these changes. In a previous study, we demonstrated that one of the earliest genes to be expressed following partial outlet obstruction in rabbits was the gene expressing stress protein-70 (HSP-70). In order to further define the genetic and molecular basis of these responses, the expression of stress gene products HSP-70 and HSP-90 in rabbit urinary bladder subjected to partial outlet obstruction has been quantitatively evaluated by Western blot coupled with laser densitometry using anti-HSP-70 and-90 monoclonal antibodies. The data show that stress gene products HSP-70 and HSP-90 are constitutively expressed in control rabbit bladder tissue and transiently increased following partial outlet obstruction. Increased content of HSP-70 was detected at 6 hr after obstruction and reached a maximum (2.7-fold over the control level) at 24 hr. Increased HSP-90 was also detected at 6 hr but reached a maximum (4.5-fold over the control level) at 12 hr. By 7 day post-obstruction, the content of these two proteins returned to the control levels. This study suggests that alterations of stress gene expression resulting in increased HSP-70 and 90 may play an important role in the response of the bladder to partial outlet obstruction.