Prevalence, the antibiogram and the frequency of virulence genes of the most predominant bacterial pathogens incriminated in calf pneumonia
Prevalence, the antibiogram and the frequency of virulence genes of the most predominant bacterial pathogens incriminated in calf pneumonia
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DOI：
10.1186/s13568-020-01037-z
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发表时间：
2020-05-29
期刊：
影响因子：
3.7
通讯作者：
Ghobashy, Madeha O., I
中科院分区：
文献类型：
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作者：
Algammal, Abdelazeem M.;El-Sayed, Mahmoud E.;Ghobashy, Madeha O., I
本研究的目的是调查引起BRD的主要细菌病原体的患病率、抗生素耐药性和某些毒力基因。共225头小牛; 55个明显健康和170患病;采样。进行细菌学检查、药敏试验和部分毒力基因的PCR检测。此外，还对E.用玻片凝集试验检测大肠杆菌。从表面健康的小牛中回收的最主要的细菌病原体是E。大肠埃希菌（16.4%）和S.金黄色葡萄球菌（10.9%）;大肠埃希氏菌（23.5%）、普通变形杆菌（12.4%）和沙门氏菌（12.4%）;金黄色葡萄球菌（11.8%）。在E.大肠埃希菌为fimH基因（100%），其次为byeaeA基因（24.5%）和hly基因（20.4%）。所有的检查。aureusstrains harboredspa和coa基因;同样，所有P.多杀性菌株携带toxA基因。大多数分离菌株对诺氟沙星和恩诺沙星敏感性较高，且E.大肠杆菌对庆大霉素、红霉素、链霉素和复方新诺明呈多重耐药。aureusandP.产气荚膜梭菌对阿莫西林、氨苄西林、四环素呈多重耐药。从埃及首次报道的肺炎小牛中获得了包括O 18、O 143、O 1和O 6在内的大肠杆菌群。总之，常规分析和基因型分析之间的协同作用是表征引起BRD的细菌病原体的有效手段。由于多药耐药菌株的发展，持续监测抗菌药物敏感性对于选择药物至关重要。
The purpose of this study was to investigate the prevalence, antibiotic resistance and certain virulence genes of the most predominant bacterial pathogens causing BRD. A total of 225 calves; 55 apparently healthy and 170 diseased; were sampled. Bacteriological examination, antimicrobial susceptibility testing and PCR based detection of some virulence genes were performed. In addition, the serotyping ofE. coliwas performed using the slide agglutination test. The most predominant bacterial pathogens retrieved from apparently healthy calves were E. coli (16.4%) and S. aureus(10.9%), and in pneumonic calves wereE. coli (23.5%),P. vulgaris(12.4%) andS. aureus(11.8%). The most prevalent virulence gene inE. coliwas thefimH gene (100%), followed byeaeA gene (24.5%) andhlygene (20.4%). All the examinedS. aureusstrains harboredspa and coa genes; likewise, allP. multocidastrains harboredtoxA gene. The majority of the isolated strains displayed remarkable sensitivity to norfloxacin and enrofloxacin; furthermore, the retrievedE. colistrains exhibited multidrug-resistance to gentamicin, erythromycin, streptomycin and trimethoprim-sulphamethoxazole, in addition, the isolatedS. aureusandP. aeruginosastrains showed multidrug-resistance to amoxicillin, ampicillin and tetracycline.E. coliserogroups including O18, O143, O1, and O6 were retrieved from pneumonic calves as the first report in Egypt. In conclusion, the synergism between the conventional and genotypic analysis is an effective gadget for the characterization of bacterial pathogens causing BRD. Continuous surveillance of antimicrobial susceptibility is essential to select the drug of choice due to the development of multidrug-resistant strains.