ε-Proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge
ε-Proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge
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DOI：
10.1111/j.1574-6968.2001.tb10968.x
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发表时间：
2001-12-18
影响因子：
2.1
通讯作者：
Prieur, D
中科院分区：
文献类型：
--
作者：
Corre, E;Reysenbach, AL;Prieur, D
原核生物系统发育多样性的样品与原位生长室部署了5天的大西洋中脊热液喷口（23度22 'N，44度57' W）。从样品中提取DNA，PCR扩增16 SrDNA。在样品中未检测到细菌。87个含有细菌16 S rDNA插入片段的克隆被选中。基于限制性片段长度多态性分析，47个克隆是独特的，然而，基于比较序列分析，其中一些是非常相似的，因此只有22个克隆被选择用于全序列和系统发育分析。细菌类型以变形杆菌门（66%）为主。其余的在变形菌门（Proteobacteria）中形成了一个新的谱系（33%）。一个克隆形成了一个独特的深分支谱系，是Aquificales的远亲。这份报告进一步扩大了越来越多的证据表明，F-变形菌是深海热液生态系统地球化学循环的重要成员，作为表生生物和自由生活的细菌参与。(C)2001年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The prokaryotic phylogenetic diversity was determined for a sample associated with an in situ growth chamber deployed for 5 days on a Mid-Atlantic Ridge hydrothermal vent (23 degrees 22'N, 44 degrees 57'W). The DNA was extracted from the sample and the 16S rDNA amplified by PCR. No Archaea were detected in the sample. Eighty-seven clones containing bacterial 16S rDNA inserts were selected. Based on restriction fragment length polymorphism analysis, 47 clones were unique, however, based on comparative sequence analysis some of these were very similar, and thus only 22 clones were selected for full sequence and phylogenetic analysis. The phylotypes were dominated by epsilon- Proteobacteria (66%). The remainder formed a novel lineage within the Proteobacteria (33%). One clone formed a distinct deeply branching lineage, and was a distant relative of the Aquificales. This report further expands the growing evidence that F-Proteobacteria are important members in biogeochemical cycling at deep-sea hydrothermal ecosystems, participating as epibionts and free living bacteria. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.