Autocrine activities of basic fibroblast growth factor: regulation of endothelial cell movement, plasminogen activator synthesis, and DNA synthesis.
Autocrine activities of basic fibroblast growth factor: regulation of endothelial cell movement, plasminogen activator synthesis, and DNA synthesis.
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基本成纤维细胞生长因子的自分泌活性：内皮细胞运动，纤溶酶原激活剂合成和DNA合成的调节。
DOI：
10.1083/jcb.107.3.1199
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发表时间：
1988-09
影响因子：
7.8
通讯作者：
Rifkin, D B
中科院分区：
文献类型：
--
作者：
Sato, Y;Rifkin, D B
我们发现，牛主动脉内皮细胞从融合区边缘的融合单层自发迁移依赖于内源性碱性成纤维细胞生长因子(BFGF)的释放。纯化的兔抗碱性成纤维细胞生长因子多克隆抗体以及亲和纯化的抗碱性成纤维细胞生长因子抗体和抗碱性成纤维细胞生长因子F(ab‘)2片段可阻断细胞运动。免疫球蛋白的抑制作用依赖于抗体浓度，是可逆的，可通过添加重组bFGF来克服，并通过bFGFSepharose柱亲和层析抗血清来去除。添加硫酸鱼精蛋白和苏拉明也可以可逆地抑制细胞运动；据报道，这两种药物可以阻断碱性成纤维细胞生长因子与其受体的结合。在损伤的单层中加入重组碱性成纤维细胞生长因子可加速细胞向裸露区域的移动。转化生长因子β已被证明可以拮抗碱性成纤维细胞生长因子的其他几种作用，它也抑制细胞运动。抗碱性成纤维细胞生长因子抗体可阻止牛毛细血管内皮细胞、BHK-21、NIH 3T3和人皮肤成纤维细胞进入裸露区域。抗碱性成纤维细胞生长因子的抗体以及苏拉明和鱼精蛋白硫酸盐也抑制了牛主动脉内皮细胞纤溶酶原激活物和DNA合成的基础水平。
We have found that the spontaneous migration of bovine aortic endothelial cells from the edge of a denuded area in a confluent monolayer is dependent upon the release of endogenous basic fibroblast growth factor (bFGF). Cell movement is blocked by purified polyclonal rabbit IgG to bFGF as well as affinity purified anti-bFGF IgG and anti- bFGF F(ab')2 fragments. The inhibitory effect of the immunoglobulins is dependent upon antibody concentration, is reversible, is overcome by the addition of recombinant bFGF, and is removed by affinity chromatography of the antiserum through a column of bFGF-Sepharose. Cell movement is also reversibly inhibited by the addition of protamine sulfate and suramin; two agents reported to block bFGF binding to its receptor. The addition of recombinant bFGF to wounded monolayers accelerates the movement of cells into the denuded area. Transforming growth factor beta which has been shown to antagonize several other effects of bFGF also inhibits cell movement. The anti-bFGF IgG prevents the movement of bovine capillary endothelial cells, BHK-21, NIH 3T3, and human skin fibroblasts into a denuded area. Antibodies to bFGF, as well as suramin and protamine sulfate also suppress the basal levels of plasminogen activator and DNA synthesis in bovine aortic endothelial cells.