MUTATION OF THE P53 TUMOR-SUPPRESSOR GENE IS NOT A FEATURE OF ENDOMETRIAL HYPERPLASIAS
MUTATION OF THE P53 TUMOR-SUPPRESSOR GENE IS NOT A FEATURE OF ENDOMETRIAL HYPERPLASIAS
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DOI：
10.1016/0002-9378(93)90644-x
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发表时间：
1993-09-01
影响因子：
9.8
通讯作者：
BERCHUCK, A
中科院分区：
文献类型：
--
作者：
KOHLER, MF;NISHII, H;BERCHUCK, A
目的：大约20%的子宫内膜癌中存在P53基因的突变和过表达。为了确定p53基因改变是否是子宫内膜癌变的早期事件，我们检测了子宫内膜增生症中的p53基因。研究设计：提取117例子宫内膜增生症(单纯性36例，复合性40例，非典型性41例)和30例子宫内膜癌的基因组脱氧核糖核酸。聚合酶链式反应扩增p53基因第5~8外显子。结果：117例子宫内膜增生症均未发现p53基因突变，而30例子宫内膜癌中有3例(10%)发生突变。经直接测序证实，3例子宫内膜癌中均存在P53基因突变(密码子157、180、272)。结论：由于P53基因突变不是子宫内膜增生症的特征，P53基因突变在子宫内膜癌的发生中可能是一个相对较晚的事件。
OBJECTIVE: Mutation and overexpression of the p53 gene occur in approximately 20% of endometrial carcinomas. To determine whether alteration of the p53 gene is an early event in endometrial carcinogenesis, we examined the p53 gene in endometrial hyperplasias.STUDY DESIGN: Genomic deoxyribonucleic acid was extracted from 117 endometrial hyperplasias (36 simple, 40 complex, 41 atypical) and 30 endometrial cancers. Exons 5 through 8 of the p53 gene were amplified by means of the polymerase chain reaction. Mutations in the p53 gene were sought with single-stranded conformation polymorphism analysis and confirmed by direct deoxyribonucleic acid sequencing.RESULTS: None of 117 endometrial hyperplasias were found to have mutations in the p53 gene, whereas mutations were seen in three of 30 (10%) endometrial cancers (p < 0.02). The p53 mutations seen in three cancers were confirmed by direct sequencing (codons 157, 180, 272).CONCLUSION: Because it does not appear to be a feature of endometrial hyperplasias, mutation of the p53 gene may represent a relatively late event in endometrial carcinogenesis.