The genomic organization and functional analysis of the promoter for the human angiotensin II type 1 receptor.
The genomic organization and functional analysis of the promoter for the human angiotensin II type 1 receptor.
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人血管紧张素 II 1 型受体启动子的基因组组织和功能分析。
DOI：
10.1006/bbrc.1994.2567
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发表时间：
1994
影响因子：
3.1
通讯作者：
Elton,TS
中科院分区：
文献类型：
--
作者：
Su,B;Martin,MM;Beason,KB;Miller,PJ;Elton,TS
我们利用5‘-RACE方法克隆并鉴定了几个人血管紧张素II 1型受体(HAT1R)的cDNA。序列分析表明，人类AT1受体至少由四个不同的mRNA转录本编码，它们共享一个相同的开放阅读框架，但它们的5‘-非翻译序列的组织结构不同。因此，即使转录了多种形式的hAT1R mRNA，它们都被翻译成相同的受体，因为这种受体的整个开放阅读框架都位于单个外显子上。这些编码5‘-非翻译序列的外显子都不以组织特异性的方式表达。通过与我们的hAT1R基因组克隆比较，确定了hAT1R基因的组织结构。人类AT1基因至少由四个外显子组成，大小至少为60kb。利用引物延伸和荧光素酶报告基因构建法将其导入人肾上腺细胞，对几个可能的启动子区域进行了鉴定。这些结果表明，我们已经克隆了hAT1R基因的功能启动子。此外，该启动子还含有肾上腺特异性反应元件。
We have cloned and characterized several human angiotensin II type 1 receptor (hAT1R) cDNAs by utilizing the 5′-RACE procedure. Sequence analysis demonstrated that human AT1 receptors are encoded by at least four distinct mRNA transcripts sharing an identical open reading frame, but differing in their organization of 5′-untranslated sequences. Therefore, even though multiple alternatively spliced forms of hAT1R mRNA are transcribed, they are all translated into identical receptors since the entire open reading frame of this receptor is harbored on a single exon. None of these exons coding for 5′-untranslated sequence is expressed in a tissue specific manner. By comparing the 5′-RACE cDNA clones with our hAT1R genomic clones, the organization of the hAT1R gene was determined. The human AT1 gene is comprised of at least four exons and spans at least 60 kb. Several putative promoter regions were characterized by utilizing primer extension and luciferase reporter gene constructs transfected into human adrenal cells. These results indicate that we have cloned a functional promoter for the hAT1R gene. Furthermore, this promoter harbors an adrenal specific response element.