Changes in expression of some two-pore domain potassium channel genes (KCNK) in selected brain regions of developing mice
Changes in expression of some two-pore domain potassium channel genes (KCNK) in selected brain regions of developing mice
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DOI：
10.1016/j.neuroscience.2007.12.011
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发表时间：
2008-02-19
期刊：
影响因子：
3.3
通讯作者：
Wisden, W.
中科院分区：
文献类型：
--
作者：
Aller, M. I.;Wisden, W.
两个P环结构域钾（K2 P或KCNK）通道产生可以影响大脑发育的递质调制的K+电流。我们通过原位杂交绘制了K2 P基因家族在发育中小鼠大脑中的表达。所有的K2 P基因有不同的表达模式，这是可能的，许多神经元类型的改变其K2 P通道亚基组成在发育过程中。与控制细胞分裂的可能作用相吻合，三个K2 P基因（r）在bar下，P结构域在a（w）在bar下，（i）在bar下，正整流（K）在bar（+）通道下，（re）在bar下，（K）在bar（+）通道下，（TREK）-1，TREK-2和（w）在坝下（i）在坝下正整流（K）在坝下（+）通道相关（a）在坝下cid-（s）在坝下敏感（K）在坝下（+）通道（任务）-2）在胚胎脑室下区和脑室区有高表达，在a（w）下bar通道（i）下bar通道（+）下bar通道（TWIK）-1，TREK-1，TREK-2和ASK-3基因在小脑外颗粒细胞层中显著表达。K2 P基因的发育表达也有一些明显的变化：例如，TREK-1在迁移后的小脑颗粒细胞中从高表达变为低表达; TREK-2在胚胎和出生后早期的任何K2 P基因的大脑中具有最高的表达之一，但转录水平在出生后时期强烈下降，小脑颗粒细胞除外。在bar（+）通道（THIK）-2基因下，ASK-1和串联孔结构域氟烷抑制（K）均特异性地在迁移后小脑颗粒细胞中开启，而例如，ASK-3基因在迁移前细胞以及迁移后细胞中强烈表达。另一方面，年轻的出生后的齿状颗粒细胞表达TWIK-1，TREK-1和TREK-2 P7前，但任务-3的表达只开始变得清晰，在这些细胞在出生后的第二周。在GABA（A）受体α 6亚基敲除小鼠小脑中，THIK-2 mRNA与ASK-1和ASK-3转录物一起上调，可能暗示THIK-2、ASK-1和ASK-3的功能关联。(c)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Two P loop domain potassium (K2P or KCNK) channels produce transmitter-modulated K+ currents that could influence brain development. We mapped by in situ hybridization the expression of the K2P gene family in the developing mouse brain. All the K2P genes had different expression patterns, and it is likely that many neuronal types change their K2P channel subunit composition during development. Fitting with a possible role in the control of cell division, three K2P genes (r) under bar andem of P domains in a (w) under bar eak (i) under bar nwardly-rectifying (K) under bar (+) channel-(re) under bar lated (K) under bar (+) channel (TREK) -1, TREK-2 and (w) under bar eak (i) under bar nwardly-rectifying (K) under bar (+) channel-related (a) under bar cid-(s) under bar ensitive (K) under bar (+) channel (TASK) -2) had high expression in the embryonic subventricular and ventricular zones, and the (t) under bar andem of P domains in a (w) under bar eak (i) under bar nwardly-rectifying (K) under bar (+) channel (TWIK) -1, TREK-1, TREK-2 and TASK-3 genes were significantly expressed in the external cerebellar granule cell layer. There were also some clear changes in developmental expression of the K2P genes: for example, TREK-1 goes from high to low expression in post-migratory cerebellar granule cells; TREK-2 has one of the highest expressions in the embryonic and early postnatal brain of any K2P gene, but transcript levels fall strongly in the postnatal periods, except for cerebellar granule cells. TASK-1 and tandem pore domain halothane-inhibited (K) under bar (+) channel (THIK) -2 genes both turn on specifically in post-migratory cerebellar granule cells, whereas the TASK-3 gene, for example, is strongly expressed in pre-migratory cells as well as post-migratory cells. On the other hand, young postnatal dentate granule cells express TWIK-1, TREK-1 and TREK-2 before P7, but TASK-3 expression only begins to become clear in these cells in the second postnatal week. THIK-2 mRNA was up-regulated with TASK-1 and TASK-3 transcripts in cerebella of GABA(A) receptor alpha 6 subunit knockout mice, possibly implying a functional association of THIK-2, TASK-1 and TASK-3. (c) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.