Differential expression of Pim-3, c-Myc, and p-p27 proteins in adenocarcinomas of the gastric cardia and distal stomach
Differential expression of Pim-3, c-Myc, and p-p27 proteins in adenocarcinomas of the gastric cardia and distal stomach
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Pim-3、c-Myc 和 p-p27 蛋白在贲门和远端胃腺癌中的差异表达
DOI：
10.1007/s13277-014-1664-z
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发表时间：
2014-05-01
期刊：
影响因子：
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通讯作者：
Xue, Liying
中科院分区：
文献类型：
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作者：
Li, Yuehong;Lou, Lei;Xue, Liying
贲门腺癌（GCA）与胃远端腺癌有着不同的病因、肿瘤特性和生物学行为。然而，其发病机制尚未完全了解。本研究旨在探讨Pim-3、c-Myc和p-p27在胃腺癌不同部位的发生、发展中的作用。应用免疫组织化学方法检测140例胃腺癌（GCA 78例，DGA 62例，正常胃组织20例）中Pim-3、c-Myc和p-p27蛋白的表达。GCA中Pim-3、c-Myc、p-p27的表达水平及Pim-3+/c-Myc+/p-p27+的共表达均显著低于DGA（P < 0.05）。免疫反应模式的详细分析表明，在DGA中，Pim-3免疫反应与肿瘤分化差，晚期肿瘤分期和淋巴结转移的存在显著相关。此外，c-Myc过表达与肿瘤分期、淋巴结转移有关，p-p27阳性表达与肿瘤低分化、分期有关。Pim-3（+）/c-Myc（+）/p-p27（+）共表达表型与肿瘤分期、淋巴结转移密切相关（P < 0.05）。而GCA中Pim-3的过表达仅与肿瘤的分化程度和分期密切相关（P < 0.05）。我们的研究结果表明Pim-3、c-Myc、p-p27和Pim-3（+）/c-Myc（+）/p-p27（+）在GCA和DGA肿瘤中的不同表达模式及其临床病理意义。我们的研究结果支持了这样一种观点，即不同的分子机制可能参与了贲门和胃远端腺癌的发展和进展。
Gastric cardia adenocarcinoma (GCA) is distinct from adenocarcinoma of the distal stomach because of its different etiological factors, tumor characteristics, and biological behavior. However, its pathogenesis is not fully understood. The purpose of this study is to characterize the role of Pim-3, c-Myc, and p-p27 in the tumorigenesis and progression of different sites of gastric adenocarcinoma by determining its pathogenetic significance. The expression of Pim-3, c-Myc, and p-p27 proteins was evaluated by immunohistochemistry in 140 resection specimens of gastric adenocarcinomas (78 GCAs , 62 DGAs and 20 normal gastric tissues). The level of expression of Pim-3, c-Myc, and p-p27 and the co-expression of all three markers (Pim-3+/c-Myc+/p-p27+) in GCA were significantly lower than that in DGA tumors (P < 0.05). Detailed analysis of the immunoreactivity patterns showed that in DGA, Pim-3 immunoreactivity was associated significantly with poor tumor differentiation, advanced tumor stage, and presence of lymph node metastasis. In addition, c-Myc overexpression correlated with tumor stage and lymph node metastasis, and positive p-p27 expression correlated with poor differentiation and tumor stage. The phenotype of Pim-3(+)/c-Myc(+)/p-p27(+) co-expression was closely correlated with tumor stage and lymph node metastasis (P < 0.05). In contrast, GCA only demonstrated a close correlation of Pim-3 overexpression with poor tumor differentiation and tumor stage (P < 0.05). Our results demonstrate the presence of different expression patterns of Pim-3, c-Myc, p-p27, and Pim-3(+)/c-Myc(+)/p-p27(+) and their clinicopathologic significance in GCA and DGA tumors. Our results add support to the notion that distinct molecular mechanisms may be involved in the development and progression of adenocarcinomas from the gastric cardia and distal portion of stomach.