Detection of DNA hybridizations using solid-state nanopores.
Detection of DNA hybridizations using solid-state nanopores.
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DOI：
10.1088/0957-4484/21/33/335102
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发表时间：
2010-08-20
期刊：
影响因子：
3.5
通讯作者：
Ling XS
中科院分区：
文献类型：
--
作者：
Balagurusamy VS;Weinger P;Ling XS
我们报道了一项利用固态纳米孔的DNA移位来检测两个双链12聚体杂交片段在单链DNA分子上的顺序排列的实验研究。样本DNA是由三个单链寡核苷酸杂交形成的三聚体分子。聚苯乙烯微珠附着在三聚体DNA的末端，提供了一种减缓移位和抑制热扩散的机制，从而允许用标准的膜片钳电子学来检测DNA的短特征。在离子流的时间轨迹中，已经成功地识别了三聚体分子通过纳米孔移位的电信号。这里报告的结果是首次成功尝试使用固态纳米孔作为离子扫描设备来解析DNA分子上的单个杂交片段(或“探针”)。
We report an experimental study of using DNA translocation through solid-state nanopores to detect the sequential arrangement of two double-stranded 12-mer hybridization segments on a single-stranded DNA molecule. The sample DNA is a trimer molecule formed by hybridizing three single-stranded oligonucleotides. A polystyrene bead is attached to the end of the trimer DNA, providing a mechanism in slowing down the translocation and suppressing the thermal diffusion, thereby allowing the detection of short features of DNA by standard patch-clamp electronics. The electrical signature of the translocation of a trimer molecule through a nanopore has been identified successfully in the temporal traces of ionic current. The results reported here represent the first successful attempt in using a solid-state nanopore as an ionic scanning device in resolving individual hybridization segments (or ‘probes’) on a DNA molecule.