Comparison of satellite cell-derived myoblasts and C2C12 differentiation in two- and three-dimensional cultures: changes in adhesion protein expression
Comparison of satellite cell-derived myoblasts and C2C12 differentiation in two- and three-dimensional cultures: changes in adhesion protein expression
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DOI：
10.1042/cbi20090335
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发表时间：
2011-02-01
影响因子：
3.9
通讯作者：
Brzoska, Edyta
中科院分区：
文献类型：
--
作者：
Grabowska, Iwona;Szeliga, Anna;Brzoska, Edyta
在单层（二维）和 3D（三维）细胞培养物中研究了参与成肌细胞分化的粘附蛋白表达的变化。在小鼠成肌细胞系 C2C12 和大鼠卫星细胞的原代培养物中检测了整合素 α3 亚基、整合素 β1 亚基、ADAM12（一种解整合素和金属蛋白酶 12）、四跨膜蛋白 CD9 和 CD81 以及 M-钙粘蛋白的表达。单层和 3D 培养物中的成肌细胞在形态和细胞骨架组织方面表现出显着差异。所有研究的蛋白质都参与了每种培养物中的成肌细胞融合，但观察到它们的表达水平存在差异。卫星细胞来源的成肌细胞比 C2C12 细胞表现出更高的粘附蛋白 mRNA 表达。此外，来自 3D 培养的 C2C12 细胞的粘附蛋白转录物表达量略高于单层培养的相同细胞。值得注意的是，发现粘附蛋白 mRNA 的水平在所有细胞培养物类型中平行变化。尽管有这一发现，但在研究体外成肌细胞分化过程时，重要的是要考虑卫星细胞来源的成肌细胞与单层和 3D 培养物中生长的细胞系 C2C12 之间的差异。
Changes in the expression of adhesion proteins involved in myoblast differentiation were investigated in monolayer (two-dimensional) and 3D (three-dimensional) cell cultures. The expression of integrin alpha3 subunit, integrin beta1 subunit, ADAM12 (a disintegrin and metalloproteinase 12), tetraspanins CD9 and CD81 and M-cadherin were examined in the murine myoblast cell line C2C12 and in a primary culture of rat satellite cells. Myoblasts in monolayer and 3D cultures showed significant differences in their morphology and cytoskeletal organization. All of the studied proteins participated in myoblast fusion in each culture examined, but differences in their levels of expression were observed. Satellite cell-derived myoblasts exhibited higher expression of adhesion protein mRNAs than C2C12 cells. Also, C2C12 cells from a 3D culture showed slightly higher expression of adhesion protein transcripts than the same cells cultured as a monolayer. Significantly, the levels of adhesion protein mRNAs were found to change in parallel in all cell culture types. Despite this finding, it is important that differences between satellite cell-derived myoblasts and cell line C2C12 grown in monolayer and 3D cultures are taken into account when studying processes of myoblast differentiation in vitro.