Allele-specific expression of the mouse B-cell surface protein CD72 on T cells.
Allele-specific expression of the mouse B-cell surface protein CD72 on T cells.
复制标题
小鼠 B 细胞表面蛋白 CD72 在 T 细胞上的等位基因特异性表达。
DOI：
10.1007/s002510050189
复制
发表时间：
1997
期刊：
影响因子：
3.2
通讯作者：
Parnes,JR
中科院分区：
文献类型：
--
作者：
Robinson,WH;Landolfi,MM;Parnes,JR
CD 72是一种分子量为45000 M的小鼠B细胞表面糖蛋白，参与B细胞的增殖和分化。 小鼠CD 72的表达被认为局限于B细胞谱系。我们最近证明，以前定义为识别小鼠淋巴细胞同种异体抗原Ly-19.2和Ly-32.2的单克隆抗体K10.6和B9.689分别识别CD 72的特异性等位基因。使用抗体介导的细胞毒性试验的早期研究表明，K10.6和B9.689与B细胞、几种T细胞系和外周T细胞亚群反应。这些发现使我们考虑CD 72也可能在T细胞亚群上表达的可能性。在这份报告中，我们证明了CD 72是组成性表达的外周血T细胞的一部分，分离自表达CD 72球蛋白的小鼠品系，但不是CD 72和CD 72细胞。在用伴刀豆球蛋白A或与板结合的CD 3特异性mAb激活T细胞后3天，CD 72在更大部分的外周T细胞以及来自表达CD 72球蛋白的小鼠品系的一部分胸腺细胞上表达。CD 72在CD 4+和CD 8+胸腺细胞和外周T细胞亚群上表达。在来自表达CD 72和CD 72细胞的小鼠品系的活化的胸腺细胞或外周T细胞上未检测到CD 72表达。通过北方印迹分析证实CD 72 mRNA表达证实了CD 72 bon外周T细胞的表达。这些结果表明，CD 72表达并不局限于表达CD 72球蛋白的小鼠品系中的B谱系细胞;相反，这些小鼠中的T谱系细胞群体也表达CD 72。
CD72 is a 45 000Mrmouse B-cell surface glycoprotein involved in B-cell proliferation and differentiation. Expression of mouse CD72 is thought to be restricted to the B-cell lineage. We recently demonstrated that the monoclonal antibodies K10.6 and B9.689, previously defined as recognizing the mouse lymphocyte alloantigens Ly-19.2 and Ly-32.2, respectively, recognize specific alleles ofCD72. Early studies using antibody-mediated cytotoxicity assays demonstrated that K10.6 and B9.689 react with B cells, several T-cell lines, and a subset of peripheral T cells. These findings led us to consider the possibility that CD72 might also be expressed on a subset of T cells. In this report we demonstrate that CD72 is constitutively expressed on a fraction of peripheral T cells isolated from strains of mice expressing theCD72ballele, but not theCD72aorCD72calleles. Three days after activating T cells with concanavalin A or plate-bound CD3-specific mAb, CD72 is expressed on a larger fraction of peripheral T cells as well as a fraction of thymocytes from mouse strains expressing theCD72ballele. CD72 is expressed on both the CD4+and CD8+thymocyte and peripheral T-cell subsets. No CD72 expression is detected on activated thymocytes or peripheral T cells from mouse strains expressing theCD72aorCD72calleles. Expression ofCD72bon peripheral T cells was confirmed by northern blot analysis demonstratingCD72mRNA expression. These results demonstrate that CD72 expression is not restricted to B lineage cells in mouse strains expressing theCD72ballele; instead, a population of T lineage cells in these mice also expresses CD72.