Visualization of Membrane Loss during the Shrinkage of Giant Vesicles under Electropulsation
Visualization of Membrane Loss during the Shrinkage of Giant Vesicles under Electropulsation
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DOI：
10.1016/j.bpj.2009.02.063
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发表时间：
2009-05-20
影响因子：
3.4
通讯作者：
Dean, David S.
中科院分区：
文献类型：
--
作者：
Portet, Thomas;Camps i Febrer, Franc;Dean, David S.
我们研究了两种不同类型的巨型单层囊泡，第一个形成的EggPC脂质和第二个形成的DOPC脂质的透化电场的效果。对两种脂质类型的囊泡的实验显示囊泡半径减小，这被解释为是由于透化过程中的脂质损失。我们表明，在尺寸上的减少可以定性地解释为脂质面积的损失，这是成比例的面积的囊泡透化。直接观察到三种可能的膜损失模式：孔形成、囊泡形成和小管形成。
We study the effect of permeabilizing electric fields applied to two different types of giant unilamellar vesicles, the first formed from EggPC lipids and the second formed from DOPC lipids. Experiments on vesicles of both lipid types show a decrease in vesicle radius, which is interpreted as being due to lipid loss during the permeabilization process. We show that the decrease in size can be qualitatively explained as a loss of lipid area, which is proportional to the area of the vesicle that is permeabilized. Three possible modes of membrane loss were directly observed: pore formation, vesicle formation, and tubule formation.