Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models.
Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models.
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DOI：
10.1038/s41598-017-01133-4
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发表时间：
2017-04-28
影响因子：
4.6
通讯作者：
Grant AJ
中科院分区：
文献类型：
--
作者：
de Vries SP;Gupta S;Baig A;Wright E;Wedley A;Jensen AN;Lora LL;Humphrey S;Skovgård H;Macleod K;Pont E;Wolanska DP;L'Heureux J;Mobegi FM;Smith DGE;Everest P;Zomer A;Williams N;Wigley P;Humphrey T;Maskell DJ;Grant AJ
弯曲杆菌是全球食源性细菌性疾病的最常见原因。肉类，特别是鸡肉在加工过程中的粪便污染是向人类传播的主要途径。缺乏对驱动C的机制的深入了解。空肠在宿主和环境中的生长和存活。本文对C.反映其生命周期各阶段的模型间空肠适应性。转座子（Tn）基因失活文库在三个C.通过Tn-插入位点测序（Tn-seq）评估了对空肠菌株的影响以及对鸡定殖期间的适应性、在家蝇中的存活以及在4 ° C营养丰富和营养贫乏的条件下的存活以及人肠上皮细胞的感染的影响。在3个C. jejuni菌株，揭示了其基因组的很大一部分致力于生长。我们报道了小说C。空肠在其整个生命周期中必不可少的因素。重要的是，我们确定了在多种条件下发挥重要作用的基因。我们的全面筛选显示了哪些鞭毛元素对生长至关重要，哪些对与宿主生物的相互作用至关重要。今后的工作重点应放在如何利用这些知识，以有效地控制由C。空肠。
Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and –poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.