NMR evidence for the existence of two native conformations of 5S RNA.
NMR evidence for the existence of two native conformations of 5S RNA.
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NMR 证据证明 5S RNA 存在两种天然构象。
DOI：
10.1093/nar/10.16.4973
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发表时间：
1982
影响因子：
14.9
通讯作者：
Moore,PB
中科院分区：
文献类型：
--
作者：
Kime,MJ;Moore,PB
用核磁共振法测定了E.大肠杆菌显示存在两种不同的分子构象异构体，其满足“A型”或天然5S RNA的操作定义。两者都很容易通过光谱与变性的“B型”5S RNA区分开来。两种A型构象异构体相互转化的条件强烈表明，它们之间的转变引起了Kao和Crothers（1）在5S RNA中首次报道的低温光学熔融转变。
NMR spectra of the non-exchangeable protons in 5S RNA from E. coli show the existence of two distinct conformers of the molecule which meet the operational definition of “A form” or native 5S RNA. Both are easily distinguished spectroscopically from denatured, “B form” 5S RNA. The conditions which interconvert the two A form conformers strongly suggest that the transition between them gives rise to the low temperature optical melting transition first reported in 5S RNA by Kao and Crothers (l).