Tumour-initiating capacity is independent of epithelial-mesenchymal transition status in breast cancer cell lines.
Tumour-initiating capacity is independent of epithelial-mesenchymal transition status in breast cancer cell lines.
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DOI：
10.1038/bjc.2014.153
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发表时间：
2014-05-13
影响因子：
8.8
通讯作者：
Chen, L.
中科院分区：
文献类型：
--
作者：
Xie, G.;Ji, A.;Yuan, Q.;Jin, Z.;Yuan, Y.;Ren, C.;Guo, Z.;Yao, Q.;Yang, K.;Lin, X.;Chen, L.
关键词：
上皮间充质转化（Epithelial-mesenchymal transition， EMT）和癌症干细胞（cancer stem cells, CSCs）被认为对癌症生物学至关重要。本研究的目的是确定EMT是否直接导致乳腺癌细胞系获得肿瘤启动能力。emt相关细胞因子刺激可诱导5株乳腺癌细胞系和1株正常乳腺细胞系上皮间质转化。在三种间充质样乳腺癌细胞系中，稳定过表达miR-200c诱导间充质-上皮转化（MET）。通过分子表达和细胞功能分析来评估EMT或MET对肿瘤启动能力和其他生物学特性的影响。EMT的诱导并没有增强肿瘤启动能力，相反，在乳腺癌细胞系上赋予CD44+/CD24−/低表型以及细胞增殖、迁移和对阿霉素和辐射的抗性。此外，MET不会导致间充质样乳腺癌细胞系的肿瘤启动能力的抑制或丧失，但它显著减弱了其他恶性特性，包括增殖、侵袭和对治疗的抵抗。上皮-间质转化不会改变乳腺癌细胞的肿瘤启动能力，但会改变一些其他生物学特性。因此，EMT和肿瘤启动能力可能在乳腺癌细胞系中没有直接联系。
Epithelial–mesenchymal transition (EMT) and cancer stem cells (CSCs) are considered to be crucial for cancer biology. The purpose of this study was to determine whether EMT directly led to the acquisition of tumour-initiating capacity in breast cancer cell lines. Epithelial–mesenchymal transition was induced in five breast cancer cell lines and one normal breast cell line by EMT-related cytokine stimulation. Mesenchymal–epithelial transition (MET) was induced by stably overexpressing miR-200c in three mesenchymal-like breast cancer cell lines. Molecular expression and cell function analysis were performed to evaluate the effect of EMT or MET on tumour-initiating capacity and other biological characteristics. The induction of EMT did not enhance tumour-initiating capacity but, instead, conferred a CD44+/CD24−/low phenotype as well as cell proliferation, migration, and resistance to doxorubicin and radiation on breast cancer cell lines. Furthermore, MET did not lead to inhibition or loss of the tumour-initiating capacity in mesenchymal-like breast cancer cell lines, but it markedly attenuated other malignant properties, including proliferation, invasion, and resistance to therapy. Epithelial–mesenchymal transition does not alter tumour-initiating capacity of breast cancer cells but some other biological characteristics. Therefore, EMT and tumour-initiating capacity may not be directly linked in breast cancer cell lines.
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影响因子：
3.7
作者：
Kong D;Banerjee S;Ahmad A;Li Y;Wang Z;Sethi S;Sarkar FH
通讯作者：
Sarkar FH
影响因子：
5.2
作者：
Leontovich AA;Zhang S;Quatraro C;Iankov I;Veroux PF;Gambino MW;Degnim A;McCubrey J;Ingle J;Galanis E;D'Assoro AB
通讯作者：
D'Assoro AB
影响因子：
3.8
作者：
Kim J;Jeong H;Lee Y;Kim C;Kim H;Kim A
通讯作者：
Kim A
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影响因子：
8
作者：
Fang, X.;Cai, Y.;Ouyang, G.
通讯作者：
Ouyang, G.
影响因子：
5.2
作者：
Fendrich, Volker;Maschuw, Katja;Konig, Alexander
通讯作者：
Konig, Alexander