KINETICS OF THE CHONDROCYTE BIOSYNTHETIC RESPONSE TO COMPRESSIVE LOAD AND RELEASE
KINETICS OF THE CHONDROCYTE BIOSYNTHETIC RESPONSE TO COMPRESSIVE LOAD AND RELEASE
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DOI：
10.1016/0304-4165(89)90067-6
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发表时间：
1989-06-27
期刊：
影响因子：
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通讯作者：
SWANN, DA
中科院分区：
文献类型：
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作者：
GRAY, ML;PIZZANELLI, AM;SWANN, DA
为了深入了解软骨组织能够感知和响应动态机械刺激的速度，我们研究了在施加和释放静态压缩应力后生物合成活性变化的时间过程。取小腿骨骺板后备区软骨，承受0、0.25和0.5 MPa的无侧限静态压应力。[35S]硫酸盐和[3H]脯氨酸的掺入量分别在加载< 1 ~ 26 h和预加载0.5、2或12 h后测定。在加载过程中，总掺入量降至稳定水平，时间常数估计为0.25 ~ 4 h（脯氨酸）和1 ~ 5 h（硫酸盐）。在释放2或12小时预负荷后3小时脯氨酸掺入超过控制水平。硫酸盐掺入在释放12小时预负荷后至少4小时保持抑制，在释放0.5和2小时预负荷后保持控制水平。我们得出结论，负荷和负荷释放对脯氨酸掺入的调制比硫酸盐掺入的调制更快。此外，卸载响应不仅仅是加载响应的倒数；这种非线性表明，对动荷载的响应不能简单地由动荷载的时间平均分量来决定。
To gain insight regarding the rate at which cartilage tissue can sense and respond to a dynamic mechnical stimulus, we have examined the time-course of changes in biosynthetic activity following both the application and release of a static compressive stress. Cartilage harvested from the reserve zone of calf epiphyseal plate was subjected to unconfined static compressive stresses of 0, 0.25 and 0.5 MPa. Incorporation of [35S]sulfate and [3H]proline was measured during loading periods of < 1 to 26 h and after preloading periods of 0.5, 2 or 12 h. During loading, total incorporation decreased to steady levels with time constants estimated to be 0.25-4 h (proline) and 1-5 h (sulfate). Proline incorporation exceeded control levels for 3 h after release of a 2 or 12 h preload. Sulfate incorporation remained depressed for at least 4 h after release of a 12 h preload and remained at control levels following release of 0.5 and 2 h preloads. We conclude that the modulation of proline incorporation by both loading and load release is faster than the modulation of sulfate incorporation. Furthermore, the response to unloading is not just the inverse of the response to loading; this nonlinearity suggests that the response to dynamic loading would not be determined simply by the time average component of the dynamic load.