BIPHASIC DOSE RESPONSE IN LOW LEVEL LIGHT THERAPY - AN UPDATE
BIPHASIC DOSE RESPONSE IN LOW LEVEL LIGHT THERAPY - AN UPDATE
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DOI：
10.2203/dose-response.11-009.hamblin
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发表时间：
2011-01-01
期刊：
影响因子：
2.5
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Huang, Ying-Ying;Sharma, Sulbha K.;Hamblin, Michael R.
自1967年以来，低水平激光（光）治疗（LLLT）就已为人所知，但由于对基本机制的不完全理解以及选择不适当的剂量测定参数导致负面研究，因此仍然存在争议。在体外研究和动物实验中均显示了LLLT的双相剂量反应或Arrhythm-Schulz曲线。这篇综述将更新我们以前（Huang et al. 2009）对这一主题的报道。LLLT的体外介质，例如三磷酸腺苷（ATP）和线粒体膜电位，显示出双相模式，而其他介质，例如线粒体活性氧簇，则显示出具有两个不同峰的三相剂量反应。活性氧（ROS）的Janus性质可能在低浓度下作为有益的信号分子，在高浓度下作为有害的细胞毒性剂，这可能部分解释了体内观察到的反应。用于小鼠创伤性脑损伤（TBI）的经颅LLLT显示出明显的双相模式，当治疗次数变化时，以及当单个治疗的能量密度变化时，观察到有益的神经效应达到峰值。进一步了解双相剂量反应在LLLT中的应用程度对于优化临床治疗是必要的。
Low-level laser (light) therapy (LLLT) has been known since 1967 but still remains controversial due to incomplete understanding of the basic mechanisms and the selection of inappropriate dosimetric parameters that led to negative studies. The biphasic dose-response or Arndt-Schulz curve in LLLT has been shown both in vitro studies and in animal experiments. This review will provide an update to our previous (Huang et al. 2009) coverage of this topic. In vitro mediators of LLLT such as adenosine triphosphate (ATP) and mitochondrial membrane potential show biphasic patterns, while others such as mitochondrial reactive oxygen species show a triphasic dose-response with two distinct peaks. The Janus nature of reactive oxygen species (ROS) that may act as a beneficial signaling molecule at low concentrations and a harmful cytotoxic agent at high concentrations, may partly explain the observed responses in vivo. Transcranial LLLT for traumatic brain injury (TBI) in mice shows a distinct biphasic pattern with peaks in beneficial neurological effects observed when the number of treatments is varied, and when the energy density of an individual treatment is varied. Further understanding of the extent to which biphasic dose responses apply in LLLT will be necessary to optimize clinical treatments.