Influence of Water Stress on the Vacuole/Extravacuole Distribution of Proline in Protoplasts of Nicotiana rustica.
Influence of Water Stress on the Vacuole/Extravacuole Distribution of Proline in Protoplasts of Nicotiana rustica.
复制标题
水分胁迫对烟草原生质体中脯氨酸液泡/液泡外分布的影响。
DOI：
10.1104/pp.72.2.590
复制
发表时间：
1983
期刊：
影响因子：
7.4
通讯作者：
H. Jäger
中科院分区：
文献类型：
--
作者：
E. Pahlich;R. Kerres;H. Jäger
通过向生长培养基中添加聚乙二醇溶液（~ 20巴）对烟草（Nicotiana rustica）植物进行胁迫。这些植物的总原生质体，液泡和液泡外组分中的脯氨酸含量和浓度已被确定，并与良好浇水的植物的原生质体和细胞组分进行比较。与对照相比，完整植株的胁迫处理导致液泡外组分中脯氨酸含量增加7倍，而液泡含量仅增加2.6倍。在对照植物的原生质体中，测得脯氨酸浓度比（液泡外与液泡）为1。在受胁迫植株的原生质体中，这一比例接近3。因此，水分胁迫似乎对脯氨酸的液泡膜定位运输系统有影响。
Tobacco (Nicotiana rustica) plants were stressed by addition of polyethylene glycol solution (-20 bar) to the growth medium. The proline contents and concentrations in total protoplasts, vacuoles, and extravacuolar fractions of these plants have been determined and compared with protoplasts and cell fractions of well-watered plants. As compared to the control, the stress treatment of intact plants results in a 7-fold increase of the proline content in the extravacuolar fraction while the vacuolar content was enriched only 2.6-fold. In protoplasts of control plants, a proline concentration ratio (extravacuole to vacuole) of 1 was measured. In protoplasts of stressed plants, this ratio was nearly 3. Thus, water stress seems to have an effect on a tonoplast-localized transport system for proline.