Corticosterone concentrations in the serum and milk of lactating rats: parallel changes after induced stress.
Corticosterone concentrations in the serum and milk of lactating rats: parallel changes after induced stress.
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DOI：
10.1210/endo-115-4-1364
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发表时间：
1984-10
期刊：
影响因子：
4.8
通讯作者：
Kwo-Yih Yeh
中科院分区：
文献类型：
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作者：
Kwo-Yih Yeh
本文研究了乙醚和戊巴比妥麻醉后泌乳大鼠血清皮质酮向乳汁的转移。麻醉应激开始后2分钟，血清和乳汁中皮质酮浓度无显著差异。到30分钟，血清中的皮质酮浓度增加到一个峰值，并在2小时的实验期间在这个水平上达到稳定，而牛奶中的浓度仅在50分钟后应激达到最大平台。血清和乳汁中皮质酮的峰值浓度分别为76 +/- 6和36 +/- 4微克/分升。通过离心超滤透析，在最大浓度下，血清和乳汁中游离皮质酮的百分比没有显著差异，因此，血清中游离皮质酮约为乳汁中游离皮质酮的200%。相反，麻醉应激对血清和乳汁皮质类固醇结合球蛋白浓度没有影响;血清与乳汁的比例约为10：1。重复乙醚应激后，牛奶中皮质酮没有积累。从乙醚应激中恢复后，乳汁皮质酮水平的下降超过了血清皮质酮水平的下降。这些结果表明，皮质酮在牛奶中的浓度迅速增加乙醚应力后，但限于显着低于血清中的水平。牛奶中的皮质酮对幼鼠发育的可能影响仍有待确定。
Transfer of corticosterone from serum to milk was studied in lactating rats after ether and pentobarbital anesthesia. Two minutes after the onset of anesthesia stress, total serum and milk corticosterone concentrations were not significantly different. By 30 min, corticosterone concentrations in serum increased to a peak and plateaued at about this level during the 2-h experimental period, while concentrations in milk reached a maximal plateau only at 50 min poststress. The peak corticosterone concentrations in serum and milk were 76 +/- 6 and 36 +/- 4 micrograms/dl, respectively. By centrifugal ultrafiltration dialysis, the percentages of free corticosterone in serum and milk were not significantly different at the maximal concentrations; thus, free corticosterone in serum was about 200% of that in milk. In contrast, anesthesia stress had no effect on serum and milk corticosteroid-binding globulin concentrations; the serum to milk ratio was approximately 10:1. No accumulation of corticosterone in milk was observed after repeated ether stress. Decreases in milk corticosterone levels after recovery from ether stress paralleled that of its serum counterpart. These results indicate that corticosterone concentrations in milk increase rapidly after ether stress, but are limited to a level significantly lower than that in serum. The possible effects of corticosterone in milk on the development of infant rats remains to be defined.