Simultaneous morphological and biochemical endogenous optical imaging of atherosclerosis
Simultaneous morphological and biochemical endogenous optical imaging of atherosclerosis
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DOI：
10.1093/ehjci/jev018
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发表时间：
2015-08-01
影响因子：
6.2
通讯作者：
Applegate, Brian E.
中科院分区：
文献类型：
--
作者：
Jo, Javier A.;Park, Jesung;Applegate, Brian E.
Aims-本研究的目的是验证新的成像技术，同时形态和生化内源性光学成像冠状动脉粥样硬化plaque.Methods和结果光学相干断层扫描（OCT）生成高分辨率的三维图像的斑块形态和内源性荧光寿命成像显微镜（FLIM）的生化组成的特点。这两种成像方式都依赖于斑块的固有光学特性，因此不需要造影剂。一个多模式OCT/FLIM系统被用来生成管腔生化地图叠加在高分辨率（7 mm轴向和13 μ m横向）的结构体积图像。对47个新鲜的死后人冠状动脉节段进行成像：病理性内膜增厚（PIT，n = 26），纤维粥样硬化（FA，n = 12），薄帽FA（TCFA，n = 2）和纤维钙化斑块（CA，n = 7），通过组织病理学确定。对多模态图像进行评价，并根据OCT专业阅片员将每个斑块识别为PIT、FA、TCFA或CA，并根据FLIM的线性判别分析将每个斑块识别为具有高脂质（HL）、高胶原（HC）或低胶原/低脂质（LCL）管腔组成。在47个斑块中，89.4%（42/47）的斑块基于OCT/FLIM评价被正确识别，使用组织组织病理学和免疫组织化学作为金标准。4例错误分类对应于混淆了PIT与HL管腔成分，即FA与HL帽。另一个对应于混淆FA与HC帽FA与LCL cap.Conclusion我们已经证明了准确的同步OCT/FLIM的冠状动脉斑块的形态和生化表征的可行性，在空间分辨率和采集速度兼容的基于导管的血管内成像。这项试验性研究的成功建立了未来多模式血管内成像系统的发展，这将有助于提高我们对斑块发病机制的理解。
Aims The aim of this study was to validate novel imaging technology for simultaneous morphological and biochemical endogenous optical imaging of coronary atherosclerotic plaque.Methods and results Optical coherence tomography (OCT) generates high-resolution 3D images of plaque morphology and endogenous fluorescence lifetime imaging microscopy (FLIM) characterizes biochemical composition. Both imaging modalities rely on plaque's intrinsic optical characteristics, making contrast agents unnecessary. A multimodal OCT/FLIM system was utilized to generate luminal biochemical maps superimposed on high-resolution (7 mmaxial and 13 mu m lateral) structural volumetric images. Forty-seven fresh postmortem human coronary segments were imaged: pathological intimal thickening (PIT, n = 26), fibroatheroma (FA, n = 12), thin-cap FA (TCFA, n = 2), and fibrocalcific plaque (CA, n = 7), determined by histopathology. Multimodal images were evaluated, and each plaque identified as PIT, FA, TCFA, or CA based on expert OCT readers, and as having high-lipid (HL), high-collagen (HC), or low-collagen/low-lipid (LCL) luminal composition based on linear discriminant analysis of FLIM. Of 47 plaques, 89.4% (42/47) of the plaques were correctly identified based on OCT/FLIM evaluation using tissue histopathology and immunohistochemistry as the gold standard. Four of the misclassifications corresponded to confusing PIT with HL luminal composition for FA with HL cap. The other corresponded to confusing FA with a HC cap for FA with an LCL cap.Conclusion We have demonstrated the feasibility of accurate simultaneous OCT/FLIM morphological and biochemical characterization of coronary plaques at spatial resolutions and acquisition speeds compatible with catheter-based intravascular imaging. The success of this pilot study sets up future development of a multimodal intravascular imaging system that will enable studies that could help improve our understanding of plaque pathogenesis.