Long-term supplementation with eicosapentaenoic acid salvages cardiomyocytes from hypoxia/reoxygenation-induced injury in rats fed with fish-oil-deprived diet
Long-term supplementation with eicosapentaenoic acid salvages cardiomyocytes from hypoxia/reoxygenation-induced injury in rats fed with fish-oil-deprived diet
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DOI：
10.1254/jjp.77.137
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发表时间：
1998-06-01
期刊：
影响因子：
--
通讯作者：
Takeo, S
中科院分区：
文献类型：
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作者：
Nasa, Y;Hayashi, M;Takeo, S
膳食补充含有二十碳五烯酸（C20：5 n-3，EPA）和二十二碳六烯酸（C22：6 n-3，DHA）的鱼油已被证明对缺血/再灌注心脏具有保护作用。我们确定了从饮食中剥夺鱼油是否自相矛盾地增强了心肌细胞对缺氧/复氧诱导的损伤的易感性，以及补充EPA或DKA是否克服了这种改变。分别用富含鱼油（FOR）的饲料、单独的鱼油剥夺（FOD）饲料、FOD饲料与EPA（1g/kg/天）或FOD饲料与DHA（1g/kg/天）喂养大鼠4周。FOD饮食减少n-3多不饱和脂肪酸（PUFA），增加n-6 PUFA，如亚油酸（C18：2）和花生四烯酸（C20：4）在心肌磷脂。补充EPA或DHA可增加其与磷脂库的结合。用胶原酶处理分离的心肌细胞进行150分钟的缺氧和随后的复氧15分钟。在FOD饮食组，缺氧和复氧后存活的杆状细胞的数量小于FOR组。补充EPA不影响杆状细胞的数量，但减弱了复氧诱导的方形细胞数量的减少。相比之下，DHA补充剂没有提供任何保护。结果表明，从膳食摄入中剥夺鱼油增强了心肌细胞对缺氧损伤的易感性，EPA而不是DKA能够挽救心肌细胞免受缺氧/复氧诱导的损伤。
Dietary supplementation of fish oil containing eicosapentaenoic acid (C20:5 n-3, EPA) and docosahexaenoic acid (C22:6 n-3, DHA) has been shown to exert protective effects on ischemic/reperfused hearts. We determined whether deprivation of fish oil from the diet paradoxically enhances susceptibility of cardiomyocytes to hypoxia/reoxygenation-induced injury and whether supplementation with either EPA or DKA overcomes such alterations. Rats were fed with fish-oil-rich (FOR) diet, fish-oil-deprived (FOD) diet alone, FOD diet with EPA (1 g/kg/day), or FOD diet with DHA (1 g/kg/day) for 4 weeks. The FOD diet reduced n-3 polyunsaturated fatty acids (PUFAs) and increased n-6 PUFAs such as linoleic (C18:2) and arachidonic acids (C20:4) in myocardial phospholipids. EPA or DHA supplementation increased its incorporation into phospholipid pools. Cardiomyocytes isolated by treatment with collagenase were subjected to 150 min of hypoxia and subsequent reoxygenation for 15 min. In the FOD diet group, the number of surviving rod-shaped cells after hypoxia and reoxygenation was smaller than that of the FOR group. Supplementation with EPA did not affect the number of rod-shaped cells, but attenuated reoxygenation-induced reduction in the number of square-shaped cells. In contrast, DHA supplementation did not afford any protection. The results suggest that deprivation of fish oil from dietary intake enhances the susceptibility of cardiomyocytes to hypoxic injury, and EPA, but not DKA, is capable of salvaging cardiomyocytes from hypoxia/reoxygenation-induced damage.