SERS-based nanostrategy for rapid anemia diagnosis.
SERS-based nanostrategy for rapid anemia diagnosis.
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DOI：
10.1039/c9nr09152a
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发表时间：
2020-01
期刊：
影响因子：
6.7
通讯作者：
P. Muhammad;S. Hanif;Jiliang Yan;F. Rehman;Jiefei Wang;Maqbool Khan;R. Chung;Albert Lee;Meng Zheng;Yuling Wang;B. Shi
中科院分区：
文献类型：
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作者：
P. Muhammad;S. Hanif;Jiliang Yan;F. Rehman;Jiefei Wang;Maqbool Khan;R. Chung;Albert Lee;Meng Zheng;Yuling Wang;B. Shi
铁检测是诊断与各种生物功能障碍相对应的多种血液相关疾病的关键标志物之一。目前可用的贫血检测方法仅可用于干扰血液中实际铁水平的预处理血液样本。当然需要具有更高灵敏度和特异性的实时检测方法来科普商业水平的临床分析。在此，我们提出了一种新的策略来确定血液中的铁，可以很容易地在商业水平上实践。众所周知的铁氰化物化学与纳米技术的混合物在全血分析中具有良好的灵敏度，无需任何预处理。该方法是一种将传统的检测方法（紫外-可见光谱法）与表面增强拉曼散射（Sers）相结合的检测系统。有机氰化物修饰的银纳米粒子（cAgNPs）可选择性地响应于Fe 3+离子和Hb蛋白，检测限分别为10 fM和0.46 μg mL-1，而不受复杂生物流体中基质干扰物种的影响。我们通过评估多发性贫血患者和正常对照的铁状态证实了我们的新cAgNPs的临床潜力。我们基于SERS的铁定量方法对于批量样品来说是非常经济实惠的，便宜，快速，灵活，并且适用于实时临床分析。这种用于金属螯合的方法具有在细胞水平上在更复杂的生命系统内进行治疗分子追踪的可扩展特征。
Iron detection is one of the critical markers to diagnose multiple blood-related disorders that correspond to various biological dysfunctions. The currently available anemia detection approach can be used only for pre-treated blood samples that interfere with the actual iron level in blood. Real-time detection approaches with higher sensitivity and specificity are certainly needed to cope with the commercial level clinical analyses. Herein, we presented a novel strategy to determine the blood iron that can be easily practiced at commercial levels. The blend of well-known iron-cyanide chemistry with nanotechnology is advantageous with ultrahigh sensitivity in whole blood analysis without any pre-treatments. This approach is a combined detection system of the conventional assay (UV-visible spectroscopy) with surface-enhanced Raman scattering (SERS). Organic cyanide modified silver nanoparticles (cAgNPs) can selectively respond to Fe3+ ions and Hb protein with a detection limit of 10 fM and 0.46 μg mL-1, respectively, without being affected by matrix interfering species in the complex biological fluid. We confirmed the clinical potential of our new cAgNPs by assessing iron-status in multiple anemia patients and normal controls. Our SERS-based iron quantitation approach is highly affordable for bulk-samples, cheap, quick, flexible, and useful for real-time clinical assays. Such a method for metal-chelation has extendable features of therapeutics molecular tracking within more complex living systems at cellular levels.