CPEB2, a novel putative translational regulator in mouse haploid germ cells
CPEB2, a novel putative translational regulator in mouse haploid germ cells
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DOI：
10.1095/biolreprod.103.015677
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发表时间：
2003-07-01
影响因子：
3.6
通讯作者：
Uesugi, S
中科院分区：
文献类型：
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作者：
Kurihara, Y;Tokuriki, M;Uesugi, S
通过胞质多聚腺苷酸化对特定mRNA的翻译控制在配子发生中具有重要作用。细胞质多聚腺苷酸化元件结合蛋白（CPEB）作为卵子发生和精子发生中的反式因子调节mRNA的细胞质多聚腺苷酸化。CPEB蛋白含有两个RNA识别基序和一个锌指结构。从基因组数据库中预测了具有相似结构的蛋白质（KIAA 0940和KIAA 1673），但对其表达和功能一无所知。在这里，我们报告了另一个新的CPEB蛋白家族成员，CPEB 2。CPEB家族成员的氨基酸序列的比较表明，该家族可以在结构上和功能上分为两组：CPEB组和CPEB 2-KIAA 0940-KIAA 1673组。CPEB 2定位于小鼠染色体5113末端，在睾丸中大量表达。然而，它被检测到逆转录聚合酶链反应在所有组织，我们检查。在转染的HeLa细胞中，它优先结合聚（U）并定位于细胞质。CPEB 2在小鼠精子发生中减数分裂后表达，表明在转录失活的单倍体精子细胞中储存的mRNA的翻译调节中可能起作用。
Translational control of specific mRNAs by cytoplasmic polyadenylation has fundamental roles in gametogenesis. The cytoplasmic polyadenylation element binding (CPEB) protein regulates cytoplasmic polyadenylation of mRNAs as a trans factor in oogenesis and spermatogenesis. The CPEB protein contains two RNA recognition motifs and a Zn-finger structure. Proteins (KIAA0940 and KIAA1673) with similar structures are predicted from the genome database, but nothing is known about their expression and function. Here, we report another novel member of the CPEB protein family, CPEB2. Comparison of the amino acid sequences of CPEB family members suggests that the family can be divided structurally and, perhaps, functionally into two groups: the CPEB group, and the CPEB2-KIAA0940-KIAA1673 group. The CPEB2 maps to mouse chromosome distal 5113 and is abundantly expressed in testis. However, it was detected by reverse transcription-polymerase chain reaction in all tissues that we examined. It preferentially binds to poly(U) and localizes to the cytoplasm in transfected HeLa cells. The CPEB2 is expressed postmeiotically in mouse spermatogenesis, suggesting a possible role in translational regulation of stored mRNAs in transcriptionally inactive haploid spermatids.