The effects of the amount of weight bearing on articular cartilage early after ACL reconstruction in rats
The effects of the amount of weight bearing on articular cartilage early after ACL reconstruction in rats
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DOI：
10.1080/03008207.2022.2141627
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发表时间：
2022-11-06
影响因子：
2.9
通讯作者：
Ozawa，Junya
中科院分区：
文献类型：
--
作者：
Kaneguchi，Akinori;Yamaoka，Kaoru;Ozawa，Junya
目的前交叉韧带（ACL）重建术后发生的骨关节炎是一个严重的问题。材料与方法将大鼠分为对照组、ACL重建组（ACLR）、ACL重建加后肢去负荷组（ACLR + HU）和ACL重建加吗啡组（ACLR + M）。各组均对右膝进行了ACL重建。为了评估负重量，在实验期间评估跑步机运动期间的单后肢站立时间比（STR;手术侧/对侧）。在第7天或14天手术后，软骨退化的内侧胫骨平台进行了组织学assessed.ResultsIn ACLR组，减少承重的特点是显着减少STR的第1天和第7天之间观察。吗啡给药可部分减轻负重。与对照组相比，ACLR组表现出Mankin评分增加，伴随着前区环氧合酶-2表达增加。在ACLR + HU组中，Mankin评分在中间和后部区域显著更高，并且这些区域的软骨厚度显著薄于ACLR组。在ACLR + M组，虽然在前区的软骨细胞密度增加，所有其他参数没有显着不同的ACLR group.ConclusionsOur结果表明，ACL重建后早期负重是重要的，以减少软骨退化。
PurposeOsteoarthritis that develops after anterior cruciate ligament (ACL) reconstruction is a critical issue. We examined the effects of the amount of weight bearing early after ACL reconstruction on articular cartilage.Materials and MethodsRats were divided into groups according to the treatment received: untreated control, ACL reconstruction (ACLR), ACL reconstruction plus hindlimb unloading (ACLR + HU), and ACL reconstruction plus morphine administration (ACLR + M). ACL reconstruction was performed on the right knee throughout the groups. To assess the amount of weight bearing, one-hindlimb standing time ratio (STR; operated side/contralateral side) during treadmill locomotion was evaluated during the experimental period. At day 7 or 14 post-surgery, cartilage degeneration of the medial tibial plateau was histologically assessed.ResultsIn the ACLR group, reduction in weight bearing characterized by significantly reduced STR was observed between day 1 and 7. Reduction in weight bearing was partially attenuated by morphine administration. Compared with the control group, the ACLR group exhibited an increased Mankin score that was accompanied by increased cyclooxygenase-2 expression in the anterior region. In the ACLR + HU group, Mankin scores were significantly higher in the middle and posterior regions, and cartilage thickness in these regions was significantly thinner than those in the ACLR group. In the ACLR + M group, although chondrocyte density in the anterior region was increased, all other parameters were not significantly different from those in the ACLR group.ConclusionsOur results suggest that early weight bearing after ACL reconstruction is important to reduce cartilage degeneration.