Presence of ghost cells and the Wnt signaling pathway in odontomas
Presence of ghost cells and the Wnt signaling pathway in odontomas
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DOI：
10.1111/j.1600-0714.2007.00550.x
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发表时间：
2007-08-01
影响因子：
3.3
通讯作者：
Kusama, Kaoru
中科院分区：
文献类型：
--
作者：
Tanaka, Akio;Okamoto, Motoyoshi;Kusama, Kaoru
背景：虽然有报道称齿瘤中存在鬼细胞，但这些细胞的产生机制尚不清楚。为了评估鬼细胞的存在和Wnt信号通路的参与，我们检测了硬角蛋白、β -连环蛋白和left -1在牙瘤中的表达。方法：应用人毛发蛋白抗体、β -连环蛋白抗体和左-1抗体对69例牙瘤进行免疫组化检测。结果：69例牙瘤中46例（66.7%）仅在鬼影细胞细胞质中表达硬角蛋白。复合牙瘤的鬼影细胞发生率（78.8%）高于复合牙瘤（29.4%）。组织病理学上，在未成熟牙釉质附近的牙源性上皮和liesegang -ring样钙化物质的中心发现鬼影细胞。在未成熟牙瘤邻近鬼影细胞的牙源性上皮细胞的细胞质和细胞核中观察到β -连环蛋白和left -1的表达。结论：这些发现提示牙瘤是一种表达硬角蛋白的肿瘤样病变，Wnt信号通路可能参与了牙瘤中鬼细胞的形成。
BACKGROUND: Although it has been reported that ghost cells are present in odontomas, the generation mechanism of these cells is unclear. To evaluate the presence of ghost cells and involvement of the Wnt signaling pathway, we examined the expression of hard keratins, beta-catenin and Lef-1 in odontomas.METHODS: Sixty-nine cases of odontoma were examined immunohistochemically with the use of antibodies against human hair proteins, beta-catenin and Lef-1.RESULTS: Expression of hard keratins was found only in the cytoplasm of ghost cells in 46 (66.7%) of the 69 odontomas. Compound odontomas (78.8%) showed a higher incidence of ghost cells than complex odontomas (29.4%). Histopathologically, ghost cells were found within odontogenic epithelium adjacent to immature enamel and in the centre of Liesegang-ring-like calcified materials. Expression of beta-catenin and Lef-1 was observed in the cytoplasm and nucleus of odontogenic epithelial cells adjacent to the ghost cells in immature odontomas.CONCLUSION: These findings suggest that odontoma is a hard keratin-expressing tumor-like lesion, and that the Wnt signaling pathway may be involved in the formation of ghost cells in odontomas.