Reassignment of sense codons in vivo
Reassignment of sense codons in vivo
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DOI：
10.1016/j.ymeth.2005.04.005
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发表时间：
2005-07-01
期刊：
影响因子：
4.8
通讯作者：
Tirrell, DA
中科院分区：
文献类型：
--
作者：
Link, AJ;Tirrell, DA
遗传密码将61个正义密码子中的一个或多个映射到一组20个典型氨基酸上。在模式生物如大肠杆菌中，正义密码子被重新分配到非标准氨基酸，这是通过操纵细胞蛋白质合成机制实现的。具体地说，对氨基酸库的控制，加上对宿主氨基酰-tRNA合成酶活性的工程，使得在常规用于表达重组蛋白的条件下，能够将正义密码子分配给各种非规范氨基酸。密码子的重新分配导致了蛋白质工程和生物有机化学的重要进展。这里我们总结了其中的一些进展，并提供了密码子重新分配的详细协议。(C)2005 Elsevier Inc.保留所有权利。
The genetic code maps one or more of the 61 sense codons onto a set of 20 canonical amino acids. Reassignment of sense codons to non-canonical amino acids in model organisms such as Escherichia coli has been achieved through manipulation of the cellular protein synthesis machinery. Specifically, control of amino acid pools, coupled with engineering of the aminoacyl-tRNA synthetase activity of the host, has enabled assignment of sense codons to a wide variety of non-canonical amino acids under conditions routinely used for expression of recombinant proteins. Codon reassignment is leading to important advances in protein engineering and bioorganic chemistry. Here we summarize some of those advances, and provide detailed protocols for codon reassignment. (c) 2005 Elsevier Inc. All rights reserved.