UHMWPE oxidation increases granulocytes activation:: a role in tissue response after prosthesis implant
UHMWPE oxidation increases granulocytes activation:: a role in tissue response after prosthesis implant
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DOI：
10.1016/s0142-9612(03)00128-5
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
Cannas, M
中科院分区：
文献类型：
--
作者：
Renò, F;Lombardi, F;Cannas, M
超高相对分子质量聚乙烯(UHMWPE)是一种广泛应用于骨科植入物的生物聚合物，在伽玛射线灭菌过程中会被氧化：这种表面氧化被认为是炎症和假体失败的主要原因。由于粒细胞参与了首次接触炎症，我们用二氢罗丹明123(DRH)用流式细胞术检测了它们的氧化爆发，以评估它们在接触正常和氧化的UHMWPE后的激活情况。外周血细胞(从裂解血中获得)负载DRH，接种到聚苯乙烯、正常和热氧化UHMWPE盘上30min后采集分析。用FSC和SSC信号对粒细胞进行个体化，并分析其细胞相关的绿色荧光。正常和氧化的超高相对分子质量聚乙烯均能刺激粒细胞的激活，平均荧光强度分别为109.3+/-3.8和150.1+/-9.2。分别)与对照样本(81.6+/-0.3)相比。此外，氧化的UHMWPE激活的粒细胞百分率(73.35+/-5.2%)显著高于未氧化的UHMWPE(21.5+/-3.8%)。UHMWPE表面氧化导致粒细胞活化增加，似乎在组织对植入物的反应中发挥了作用。(C)2003爱思唯尔科学有限公司。保留所有权利。
Ultra-high molecular weight polyethylene (UHMWPE), a biopolymer widely used in orthopaedic implants, is oxidized during gamma-ray sterilization: such surface oxidation is considered as major responsible for inflammation and prosthesis failure. As granulocytes are involved in first contact inflammation, we have measured their oxidative burst by flow cytometry using dihydrorhodamine 123 (DRH) to evaluate their activation following contact with normal and oxidized UHMWPE. Peripheral blood cells (obtained by lysed blood) were loaded with DRH, seeded onto polystyrene, normal and heat-oxidized UHMWPE disks for 30 min and then collected for analysis. Granulocytes were individuated using FSC and SSC signals and their cell associated green fluorescence was analyzed. Both normal and oxidized UHMWPE stimulated granulocytes activation as showed by the mean fluorescence emitted (109.3 +/- 3.8 and 150.1 +/- 9.2. respectively) compared to control samples (81.6 +/- 0.3). Moreover oxidized UHMWPE activated a significantly higher percentage of granulocytes (73.35 +/- 5.2%) compared to not-oxidized UHMWPE (21.5 +/- 3.8%). UHMWPE surface oxidation responsible for increased granulocyte activation seems to play a role in tissue response to implants. (C) 2003 Elsevier Science Ltd. All rights reserved.