Complete nucleotide sequence of the mitochondrial genome of a Malagasy poison frog Mantella madagascariensis:: Evolutionary implications on mitochondrial genomes of higher anuran groups
Complete nucleotide sequence of the mitochondrial genome of a Malagasy poison frog Mantella madagascariensis:: Evolutionary implications on mitochondrial genomes of higher anuran groups
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DOI：
10.1016/j.ympev.2005.11.021
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发表时间：
2006-04-01
影响因子：
4.1
通讯作者：
Hasegawa, M
中科院分区：
文献类型：
--
作者：
Kurabayashi, A;Usuki, C;Hasegawa, M
我们测定了马达加斯加毒蛙Mantella madagascar （Mantellidae）线粒体基因组的完整核苷酸序列，以及两个Mantella （M. baroni和All）的部分序列。bernhardi)和另外两个mantellid物种（Boophis madagascar和Mantidactylus cf. ulcerosus）。在迄今所研究的所有脊椎动物mtdna中，马达加斯加m.d ascariensis基因组被证明是最大的（23 kbp）。结果表明：(1)与脊椎动物和其他无尾动物的线粒体基因组相比，部分基因和基因区域的位置发生了重排；(2)编码了两个不同的基因和一个与蛋氨酸转移RNA基因（IRNA-Met）对应的假基因；(3)存在两个序列同源性很高的控制区。这些特征为另外两个曼特拉属共有，而其他曼特拉属没有，表明在曼特拉属分化之前，在一个共同祖先谱系中发生了动态的基因组重组。重组途径可以用基因复制和缺失模型来解释。重复和缺失事件似乎也导致了Mantella mt基因组控制区的协调序列进化。这也提示在Mantella mt基因组中长期存在的伪tRNA-Met基因可能是NADH脱氢酶亚单位（ND） 2 mRNA加工的标点符号。利用mt基因的大序列数据集进行系统发育分析，支持了Mantellidae和Rhacophoridae的单系性以及其他最近的蛙类系统发育观点。由此产生的系统发育关系也表明两个tRNA-Met基因平行出现，重复的控制区和ND5基因易位在独立的牛科谱系中。(c) 2005爱思唯尔公司版权所有。
We determined the complete nucleotide sequence of the mitochondrial (mt) genome of a Malagasy poison frog, Mantella madagaseariensis (family Mantellidae), and partial sequences of two Mantella (M. baroni and All. bernhardi) and two additional mantellid species (Boophis madagascariensis and Mantidactylus cf. ulcerosus). The M. madagascariensis genome was shown to be the largest (23 kbp) of all vertebrate mtDNAs investigated so far. Furthermore, the following unique features were revealed: (1) the positions of some genes and gene regions were rearranged compared to mitochondrial genomes typical for vertebrates and other anuran groups, (2) two distinct genes and a pseudogene corresponding to transfer RNA gene for methionine (IRNA-Met) were encoded, and (3) two control regions with very high sequence homology were present. These features were shared by the two other Mantella species but not the other mantellid species, indicating dynamic genome reorganization in a common ancestor linage before divergence of the Mantella genus. The reorganization pathway could be explained by a model of gene duplication and deletion. Duplication and deletion events also seem to have been responsible for concerted sequence evolution of the control regions in Mantella mt genomes. It is also suggested that the pseudo tRNA-Met gene sustained for a long time in Mantella mt genomes possibly functions as a punctuation marker for NADH dehydrogenase subunit (ND) 2 mRNA processing. Phylogenetic analyses employing a large sequence data set of mt genes supported the monophyly of Mantellidae and Rhacophoridae and other recent phylogenetic views for ranoid frogs. The resultant phylogenetic relationship also suggested parallel Occurrence of two tRNA-Met genes, duplicated control regions, and ND5 gene translocation in independent ranoid lineages. (c) 2005 Elsevier Inc. All rights reserved.