Application of 3-D Microfluidic Models for Studying Mass Transport Properties of the Tumor Interstitial Matrix
Application of 3-D Microfluidic Models for Studying Mass Transport Properties of the Tumor Interstitial Matrix
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DOI：
10.3389/fbioe.2019.00006
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发表时间：
2019-01-23
影响因子：
5.7
通讯作者：
Song, Jonathan W.
中科院分区：
文献类型：
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作者：
Avendano, Alex;Cortes-Medina, Marcos;Song, Jonathan W.
与癌症进展相关的物理重塑导致肿瘤间质空间中的物质转运障碍。这种阻碍最终影响了控制细胞命运和癌症治疗效力的大分子的分布。因此，了解特定的细胞外基质（ECM）和细胞成分如何调节肿瘤组织中的转运，可能会导致改善患者预后的基质正常化策略。过去几十年的研究通过表征两个控制参数提供了对肿瘤间质转运的定量见解：（1）分子扩散率和（2）水力传导率。然而，许多用于测量这些参数的常规技术是有限的，因为它们不能通过实验操纵肿瘤的物理和细胞环境。在这里，我们研究微流体系统的应用和未来的机会，以确定在肿瘤细胞外基质中的质量运输的理化介质。进一步发展和采用微流体系统来量化肿瘤转运参数，有可能将基础科学与转化研究联系起来，以推进肿瘤学中的个性化医学。
The physical remodeling associated with cancer progression results in barriers to mass transport in the tumor interstitial space. This hindrance ultimately affects the distribution of macromolecules that govern cell fate and potency of cancer therapies. Therefore, knowing how specific extracellular matrix (ECM) and cellular components regulate transport in the tumor interstitium could lead to matrix normalizing strategies that improve patient outcome. Studies over the past decades have provided quantitative insights into interstitial transport in tumors by characterizing two governing parameters: (1) molecular diffusivity and (2) hydraulic conductivity. However, many of the conventional techniques used to measure these parameters are limited due to their inability to experimentally manipulate the physical and cellular environments of tumors. Here, we examine the application and future opportunities of microfluidic systems for identifying the physiochemical mediators of mass transport in the tumor ECM. Further advancement and adoption of microfluidic systems to quantify tumor transport parameters has potential to bridge basic science with translational research for advancing personalized medicine in oncology.