Comparison of Shedding, and Antibiotic Resistance Properties of Listeria monocytogenes Isolated From Milk, Feces, Urine, and Vaginal Secretion of Bovine, Ovine, Caprine, Buffalo, and Camel Species in Iran
Comparison of Shedding, and Antibiotic Resistance Properties of Listeria monocytogenes Isolated From Milk, Feces, Urine, and Vaginal Secretion of Bovine, Ovine, Caprine, Buffalo, and Camel Species in Iran
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DOI：
10.5812/jjm.6616
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发表时间：
2013-01-01
影响因子：
0.6
通讯作者：
Dehkordi, Shahin Nejat
中科院分区：
文献类型：
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作者：
Dehkordi, Farhad Safarpoor;Barati, Sara;Dehkordi, Shahin Nejat
背景：单核细胞增多性李斯特菌可引起以脑炎、败血症、流产或死胎为特征的脑膜炎。传统的诊断方法是通过血清学反应，而近年来发展了多种分子生物学方法，以提高检测的安全性、快速性、灵敏性和准确性。流行病学，患病率，脱落途径，和抗生素耐药性的特点。目的：本研究旨在研究单核细胞增生性李斯特菌在不同动物中的散毒途径和耐药性。从伊朗的牛、绵羊、山羊、布法罗和骆驼的牛奶、粪便、尿液和阴道分泌物中分离出单核细胞增多症。通过培养、常规PCR和实时荧光PCR检测样品中是否存在L。单核细胞增多症结果：2180份标本中，细菌培养阳性186份，阳性率为8.53%。单核细胞增多症其中牛奶、粪便、阴道拭子和尿液标本中检出阳性的分别为61份（10.23%）、40份（6.46%）、43份（9.7%）和48份（9.19%）。单核细胞增多症，使用常规PCR。经荧光定量PCR检测，牛奶、粪便、阴道拭子和尿液中分别有69份（11.57%）、48份（7.75%）、53份（11.96%）和57份（10.91%）阳性。单核细胞增多症。常规PCR和实时荧光定量PCR的敏感性和特异性分别为94%和99.1%，100%和97.9%。L.单核细胞增多症在牛乳（10.83%）、羊尿（16.98%）、山羊粪（14.38%）、布法罗乳（11.11%）和骆驼阴道分泌物（15.18%）中的脱落率最高。对四环素的耐药率最高（71.3%），对呋喃妥因的耐药率最低（5.72%）。单核细胞增多症在不同的动物种类和不同的样品中是不同的。由于L.因此，兽医应多加注意，开抗生素。我们建议使用实时荧光PCR检测L。在临床样品中的单核细胞增多症，并使用磁盘扩散方法，以处方合适的抗生素。
Background: Listeria monocytogenes causes listeriosis characterized by encephalitis, septicaemia, and abortion or stillbirth. Its traditional diagnosis is based on serological responses, whileseveral molecular methods have been developed for safer and more rapid, sensitive, and accurate detection. The epidemiology, prevalence, shedding routes, and antibiotic resistance properties of L. monocytogenes are essentially unknown in various animals species.Objectives: The present study was performed to study the shedding routes, and antibiotic resistance properties of L. monocytogenes isolated from milk, feces, urine, and vaginal secretion of bovine, ovine, caprine, buffalo, and camel in Iran.Materials and Methods: A total of 596 milk, 619 feces, 443 vaginal swab, and 522 urine samples were collected from various animal species. Samples were examined by culture, conventional and real-time PCR for evaluating the presence of L. monocytogenes. Finally antimicrobial resistance properties were studied using the simple disc diffusion method.Results: The culture method showed that 186 of 2180 samples (8.53%) had positive results for L. monocytogenes. In total, 61 (10.23%) milk, 40 (6.46%) feces, 43 (9.7%) vaginal swab, and 48 (9.19%) urine samples had positive results for L. monocytogenes using conventional PCR. After the Light Cycler real-time PCR it was recognized that 69 (11.57%) milk, 48 (7.75%) feces, 53 (11.96%) vaginal swab and 57 (10.91%) urine samples hadpositive results for the presence of L. monocytogenes. The sensitivity and specificity of conventional and real-time PCR were 94% and 99.1%, and 100% and 97.9%, respectively. L. monocytogenes had the highest shedding in bovine milk (10.83%), ovine urine (16.98%), caprine feces (14.38%), buffalo milk (11.11%), and camel vaginal secretion (15.18%). Antibiotic resistance to tetracycline (71.3%) was the highest, while the resistance to nitrofurantoin (5.72%) had the lowest frequency.Conclusions: Shedding of L. monocytogenes in different animal species, and different samples are different. Due to antibiotic resistance, especially in L. monocytogenes, veterinarians should pay more attention to prescribe antibiotics. We recommend using the real-time PCR for safe, sensitive, and rapid detection of L. monocytogenes in clinical samples, and using the disk diffusion methods to prescribe suitable antibiotics.