Infection of cynomolgus monkeys with HIV-2 protects against pathogenic consequences of a subsequent simian immunodeficiency virus infection.
Infection of cynomolgus monkeys with HIV-2 protects against pathogenic consequences of a subsequent simian immunodeficiency virus infection.
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食蟹猴感染 HIV-2 可以防止随后的猿猴免疫缺陷病毒感染的致病后果。
DOI：
10.1097/00002030-199008000-00010
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发表时间：
1990
期刊：
影响因子：
3.8
通讯作者：
Gunnel Biberfeld
中科院分区：
文献类型：
--
作者：
P. Putkonen;Rigmor Thorstensson;Jan Albert;Kerstin Hild;Erling Norrby;Peter Biberfeld;Gunnel Biberfeld
食蟹猴的猴免疫缺陷病毒（SIV）感染导致严重的免疫缺陷，具有致死性结局。相比之下，HIV-2感染这些灵长类动物并没有明显引起任何免疫异常。在这项研究中，三只食蟹猴实验性感染HIV-2株SBL-K135，168天后用10-100动物感染剂量的密切相关的SIV株SM进行攻击，以研究保护性免疫。在SIV攻毒时，HIV-2感染猴具有抗HIV-2的中和抗体，但病毒不能再从其外周血单核细胞（PBMC）中回收，且未观察到临床症状或CD 4+淋巴细胞减少。用SIV攻击后9个月的随访表明，HIV-2感染猴对SIV诱导的免疫缺陷（CD 4+淋巴细胞不减少）和淋巴结病有保护作用。然而，他们对SIV感染没有抵抗力，因为病毒可以从他们的PBMC中回收，并且他们产生了记忆抗体应答。4只接种相同剂量SIV的幼稚对照猴持续感染，CD 4+细胞绝对数量减少，并显示出明显的淋巴结病。四只对照动物中有两只在感染后58-265天死于免疫抑制疾病。免疫组化检查显示，丰富的病毒抗原在淋巴结活检从SIV感染的对照猴，但缺乏SIV或HIV-2抗原的活检从三个HIV-2预感染和SIV-超感染的猴子。目前的研究表明，诱导免疫的可能性，对免疫缺陷引起的灵长类慢病毒，一个概念，也适用于人类的HIV感染和艾滋病。
Simian immunodeficiency virus (SIV) infection in cynomolgus macaques leads to severe immunodeficiency with a fatal outcome. In contrast, HIV-2 infects these primates without apparently causing any immunological abnormalities. In this study three cynomolgus monkeys were experimentally infected with HIV-2 strain SBL-K135 and 168 days later challenged with 10-100 animal infectious doses of the closely related SIV strain SM to study protective immunity. At the time of SIV challenge the HIV-2-infected monkeys had neutralizing antibodies against HIV-2, but virus could no longer be recovered from their peripheral blood mononuclear cells (PBMCs) and no clinical symptoms or decrease in CD4+ lymphocytes were observed. Follow-up for 9 months after challenge with SIV showed that the HIV-2-infected monkeys were protected against SIV-induced immunodeficiency (no decrease of CD4+ lymphocytes) and lymphadenopathy. However, they were not resistant to SIV infection since virus could be recovered from their PBMCs and they developed anamnestic antibody responses. Four naive control monkeys which were inoculated with the same dose of SIV became persistently infected and developed a decrease of the absolute numbers of CD4+ cells and showed a marked lymphadenopathy. Two out of four control animals died 58-265 days postinfection with an immunosuppressive disease. Immunohistochemical examination showed abundant viral antigen in lymph-node biopsies from the SIV-infected control monkeys but absence of SIV or HIV-2 antigens in the biopsies from the three HIV-2-preinfected and SIV-superinfected monkeys. The present study demonstrates possibilities for induction of immunity against immunodeficiency induced by a primate lentivirus, a concept with application also to HIV infection and AIDS in man.