The SCID mouse: an experimental model for endometriosis
The SCID mouse: an experimental model for endometriosis
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DOI：
10.1093/humrep/14.12.3107
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发表时间：
1999-12-01
影响因子：
6.1
通讯作者：
Isaacson, K
中科院分区：
文献类型：
--
作者：
Awwad, JT;Sayegh, RA;Isaacson, K
本研究旨在通过确定诱导的子宫内膜植入物的形态学和组织学特征，并描述这些植入物的特定生化特性，来验证重症联合免疫缺陷（SCID）小鼠作为子宫内膜异位症实验模型的适用性。将人分泌期子宫内膜组织注射到SCID/SCID CB17成年雌性小鼠的腹腔中。在57只SCID小鼠中的55只（96.5%）观察到成功的腹腔植入，表现为局限性隆起结节。植入病变的苏木精 - 伊红染色显示在间质和炎症细胞混合的背景下存在子宫内膜腺组织。当给小鼠施用孕酮时，上皮腺发生明确的分泌变化。使用多克隆人广谱细胞角蛋白抗体进行的免疫组织化学分析显示，人植入物的腺上皮选择性阳性染色，周围间质无染色。使用补体成分3 cDNA放射性标记的核糖核酸探针进行的原位杂交分析显示，与间质相比，腺体中的信号明显更强。由于SCID小鼠中的人子宫内膜植入物显示保留特定的组织学、功能和生化特性，我们得出结论，SCID小鼠是研究子宫内膜异位症的一种有吸引力的动物模型。
The purpose of this study was to validate the suitability of the severe combined immunodeficient (SCID) mouse as an experimental model for endometriosis, by defining the morphological and histological features of induced endometrial implants, and characterizing specific biochemical properties of these implants. Human secretory endometrial tissues were injected into the peritoneal cavity of SCID/SCID CB17 mature female mice. Successful peritoneal implantation was observed in 55 of 57 (96.5%) SCID mice and consisted of circumscribed elevated nodules, Haematoxylin-eosin staining of implanting lesions demonstrated the presence of endometrial glandular tissue in a mixed background of stromal and inflammatory cells, When progesterone was administered to mice, epithelial glands underwent well-defined secretory changes. Immunohistochemical analysis using polyclonal human pan-cytokeratin antibodies demonstrated selective positive staining in the glandular epithelium of the human implants with none in the surrounding stroma. In-situ hybridization analysis using complement component 3 cDNA radiolabelled riboprobes yielded significantly more intense signals in glands compared to stroma, As human endometrial implants in SCID mice were shown to retain specific histological, functional and biochemical properties, we conclude that the SCID mouse is an attractive animal model for the study of endometriosis.