Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner
Display of heterologous antigens on the Bacillus subtilis spore coat using CotC as a fusion partner
复制标题
DOI：
10.1016/j.vaccine.2003.09.031
复制
发表时间：
2004-03-12
期刊：
影响因子：
5.5
通讯作者：
Cutting, SM
中科院分区：
文献类型：
--
作者：
Mauriello, EMF;Duc, LH;Cutting, SM
我们报告了使用 CotC（枯草芽孢杆菌孢子衣的主要成分）作为在孢子衣上表达两种异源抗原的融合伴侣。表达破伤风梭菌破伤风毒素片段 C (TTFC) 或大肠杆菌 (LTB) 不耐热毒素 B 亚基的重组孢子用于口服给药，并在小鼠模型中显示出产生特异性的全身和粘膜免疫反应。该报告通过与 CotB 融合扩展了先前描述的 TTFC 在孢子表面的表达 [J Bacteriol 183 (2001) 6294] 及其用于口服疫苗的用途 [Infect Immun 71 (2003) 2810]，表明不同的抗原可以成功地呈递在孢子外皮上，并支持使用孢子作为粘膜免疫的有效载体。 (C) 2003 Elsevier Ltd. 保留所有权利。
We report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of two heterologous antigens on the spore coat. Recombinant spores expressing tetanus toxin fragment C (TTFC) of Clostridium tetani or the B subunit of the heat-labile toxin of Escherichia coli (LTB) were used for oral dosing and shown to generate specific systemic and mucosal immune responses in a murine model. This report, expanding the previously described expression of TTFC on the spore surface by fusion to CotB [J Bacteriol 183 (2001) 6294] and its use for oral vaccination [Infect Immun 71 (2003) 2810] shows that different antigens can be successfully presented on the spore coat and supports the use of the spore as an efficient vehicle for mucosal immunisation. (C) 2003 Elsevier Ltd. All rights reserved.