Metabolism of DNA secondary structures at the eukaryotic replication fork
Metabolism of DNA secondary structures at the eukaryotic replication fork
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DOI：
10.1016/j.dnarep.2014.03.016
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发表时间：
2014-07-01
期刊：
影响因子：
3.8
通讯作者：
Boulton, Simon J.
中科院分区：
文献类型：
--
作者：
Leon-Ortiz, Ana Maria;Svendsen, Jennifer;Boulton, Simon J.
DNA二级结构在很大程度上有利于许多细胞过程，但可能对复制机制的进展构成特定的威胁，从而对基因组复制和细胞分裂构成威胁。一些专门的酶可以分解这些结构，使复制分叉进程能够忠实地进行。在这篇综述中，我们讨论了导致在真核生物中鉴定这些酶的体外和体内数据，以及表明它们特异性地作用于复制分叉来解析二级结构的证据。我们专注于螺旋酶的作用，它催化核苷酸复合体的解离，以及核酸酶的作用，它裂解二级结构，允许复制分叉进行，以牺牲局部重排为代价。最后，我们讨论在拆除DNA二级结构方面的悬而未决的问题，以及作用于特定类型结构的不同酶之间的相互作用。(C)2014爱思唯尔B.V.保留所有权利。
DNA secondary structures are largely advantageous for numerous cellular processes but can pose specific threats to the progression of the replication machinery and therefore genome duplication and cell division. A number of specialized enzymes dismantle these structures to allow replication fork progression to proceed faithfully. In this review, we discuss the in vitro and in vivo data that has lead to the identification of these enzymes in eukaryotes, and the evidence that suggests that they act specifically at replication forks to resolve secondary structures. We focus on the role of helicases, which catalyze the dissociation of nucleotide complexes, and on the role of nucleases, which cleave secondary structures to allow replication fork progression at the expense of local rearrangements. Finally, we discuss outstanding questions in terms of dismantling DNA secondary structures, as well as the interplay between diverse enzymes that act upon specific types of structures. (C) 2014 Elsevier B.V. All rights reserved.