Phenotypic and genetic variation in phosphorus-deficiency-tolerance traits in Chinese wheat landraces
Phenotypic and genetic variation in phosphorus-deficiency-tolerance traits in Chinese wheat landraces
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中国小麦地方品种耐缺磷性状的表型和遗传变异
DOI：
10.1186/s12870-020-02492-3
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发表时间：
2020-07-13
影响因子：
5.3
通讯作者：
Liu, Yaxi
中科院分区：
文献类型：
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作者：
Lin, Yu;Chen, Guangdeng;Liu, Yaxi
背景磷缺乏是影响全球作物生产的主要限制因素。为了了解耐缺磷性的遗传变异，对707个中国小麦地方品种在施磷（AP）和不施磷（NP）下的15个幼苗性状进行了评价。采用18,594个单核苷酸多态性和38,678个多样性阵列技术测序标记来检测AP和NP下标记与性状的关联。结果从707个中国小麦地方品种中筛选出耐受性最高的前10个基因型和敏感性最低的10个基因型，用于未来的育种和遗传分析。通过 CMLM 和 SUPER 方法，总共 13 个性状的 55 个显着标记（81 个标记-性状关联）。这些分布在染色体1A、1B、2A、2B、2D、3A、4B、5A、5B、6A、6B、6D、7A和7B上。考虑到连锁不平衡衰减距离，在AP和NP下分别检测到25个和12个数量性状位点（QTL）（其中9个QTL为NP特有）。结论极其耐受的地方品种可用于选育耐缺磷品种。通过标记辅助选择，QTL 可用于小麦育种。我们的研究结果为耐缺磷的遗传分析提供了新的见解，并将有助于选育耐缺磷品种。
BackgroundPhosphorus deficiency is a major limiting factors for affecting crop production globally. To understand the genetic variation of phosphorus-deficiency-tolerance, a total of 15 seedling traits were evaluated among 707 Chinese wheat landraces under application of phosphorus (AP) and non-application of phosphorus (NP). A total of 18,594 single-nucleotide polymorphisms and 38,678 diversity arrays technology sequencing markers were used to detect marker-trait associations under AP and NP.ResultsTop ten genotypes with extremely tolerance and bottommost ten genotypes with extremely sensitivity were selected from 707 Chinese wheat landraces for future breeding and genetic analysis. A total of 55 significant markers (81 marker-trait associations) for 13 traits by both CMLM and SUPER method. These were distributed on chromosomes 1A, 1B, 2A, 2B, 2D, 3A, 4B, 5A, 5B, 6A, 6B, 6D, 7A and 7B. Considering the linkage disequilibrium decay distance, 25 and 12 quantitative trait loci (QTL) were detected under AP and NP, respectively (9 QTL were specific to NP).ConclusionsThe extremely tolerant landraces could be used for breeding phosphorus-deficiency-tolerant cultivars. The QTL could be useful in wheat breeding through marker-assisted selection. Our findings provide new insight into the genetic analysis of P-deficiency-tolerance, and will be helpful for breeding P-deficiency-tolerant cultivars.