Symbiont-induced changes in host actin during the onset of a beneficial animal-bacterial association
Symbiont-induced changes in host actin during the onset of a beneficial animal-bacterial association
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DOI：
10.1128/aem.70.3.1434-1441.2004
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发表时间：
2004-03-01
影响因子：
4.4
通讯作者：
McFall-Ngai, MJ
中科院分区：
文献类型：
--
作者：
Kimbell, JR;McFall-Ngai, MJ
细菌对动物细胞骨架的影响仅在致病性关联方面得到了广泛的研究。我们的特点是宿主细胞骨架肌动蛋白的变化引起的细菌合作伙伴在发病期间的合作动物-细菌协会使用鱿鱼弧菌模型。二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹分析表明，费氏弧菌诱导的细菌相关的宿主组织中的肌动蛋白丰度的发病过程中的共生显着增加。免疫细胞化学显示，这种肌动蛋白丰度的变化与肌动蛋白在上皮内衬导管的顶端细胞表面增加2 - 3倍相关，这是共生体进入宿主组织的途径。实时逆转录聚合酶链反应和原位杂交未检测到肌动蛋白mRNA的相应变化。与细菌诱导的肌动蛋白水平变化时间相关的是导管周长减少2 - 3倍，与导管管腔交界的细胞平均数量减少20%，导管细胞形状发生显著变化。当考虑与以前的研究的生物力学和生化特性的管道，这些研究结果表明，细菌共生体，殖民宿主器官后，诱导修改，物理和化学限制的机会，随后的殖民者通过管道。继续研究鱿鱼弧菌系统将允许进一步比较致病菌和合作细菌影响宿主细胞中细胞骨架动态的机制。
The influence of bacteria on the cytoskeleton of animal cells has been studied extensively only in pathogenic associations. We characterized changes in host cytoskeletal actin induced by the bacterial partner during the onset of a cooperative animal-bacteria association using the squid-vibrio model. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis revealed that Vibrio fischeri induced a dramatic increase in actin protein abundance in the bacteria-associated host tissues during the onset of the symbiosis. Immunocytochemistry revealed that this change in actin abundance correlated with a two- to threefold increase in actin in the apical cell surface of the epithelium-lined ducts, the route of entry of symbionts into host tissues. Real-time reverse transcriptase PCR and in situ hybridization did not detect corresponding changes in actin mRNA. Temporally correlated with the bacteria-induced changes in actin levels was a two- to threefold decrease in duct circumference, a 20% loss in the average number of cells interfacing with the duct lumina, and dramatic changes in duct cell shape. When considered with previous studies of the biomechanical and biochemical characteristics of the duct, these findings suggest that the bacterial symbionts, upon colonizing the host organ, induce modifications that physically and chemically limit the opportunity for subsequent colonizers to pass through the ducts. Continued study of the squid-vibrio system will allow further comparisons of the mechanisms by which pathogenic and cooperative bacteria influence cytoskeleton dynamics in host cells.