Characterization of boscalid-induced oxidative stress and neurodevelopmental toxicity in zebrafish embryos
Characterization of boscalid-induced oxidative stress and neurodevelopmental toxicity in zebrafish embryos
复制标题
啶酰菌胺诱导斑马鱼胚胎氧化应激和神经发育毒性的表征。
DOI：
10.1016/j.chemosphere.2019.124753
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发表时间：
2020-01-01
期刊：
影响因子：
8.8
通讯作者：
Lu, Huiqiang
中科院分区：
文献类型：
--
作者：
Wang, Honglei;Meng, Zhen;Lu, Huiqiang
啶酰菌胺是一种广泛应用于农业的杀菌剂，在环境和农产品中经常被检测到。然而，关于啶酰菌胺神经毒性作用的证据很少。本研究以斑马鱼为动物模型，探讨啶酰菌胺对水生脊椎动物或高等动物的毒性作用及其机制。我们揭示了啶酰菌胺诱导与氧化应激相关的发育缺陷。在48 hpf下暴露于啶酰菌胺后，观察到斑马鱼胚胎的发育缺陷，包括头部畸形、色素减退、新生神经元数量减少、脑室周围结构缺陷、脑细胞间隙扩大和核浓缩。有趣的是，我们发现啶酰菌胺可能直接诱导氧化应激并改变ATP酶的活性，从而破坏参与神经发育和递质传递信号以及黑素细胞分化和黑素合成信号的基因的表达。最终，神经细胞和黑色素细胞的分化都受到影响，黑色素的合成受到抑制，导致形态异常。此外，暴露于啶酰菌胺导致更少的和不平衡的运动和改变的倾向，在仔鱼的运动。本研究结果为全面评价其毒性及对环境和农产品中残留的预警提供了新的依据。(C)2019爱思唯尔有限公司版权所有。
Boscalid is a widely used fungicide in agriculture and has been frequently detected in both environments and agricultural products. However, evidence on the neurotoxic effect of boscalid is scarce. In this study, zebrafish served as an animal model to investigate the toxic effects and mechanisms of boscalid on aquatic vertebrates or higher animals. And we unravelled that boscalid induced developmental defects associated with oxidative stress. Developmental defects, including head deformity, hypopigmentation, decreased number of newborn neurons, structural defects around the ventricle, enlarged intercellular space in the brain, and nuclear concentration, were observed in zebrafish embryos after boscalid exposure at 48 hpf. Interestingly, we found that boscalid might directly induce oxidative stress and alter the activity of ATPase, which in turn disrupted the expression of genes involved in neurodevelopment and transmitter-transmitting signalings and melanocyte differentiation and melanin synthesis signalings. Ultimately, the differentiation of nerve cells and melanocytes were both impacted and the synthesis of melanin was inhibited, leading to morphological abnormalities. Additionally, exposure to boscalid led to less and imbalance motion and altered tendency of locomotor in larval fish. Collectively, our results provide new evidences for a comprehensive assessment of its toxicity and a warning for its residues in environment and agricultural products. (C) 2019 Elsevier Ltd. All rights reserved.