Effects of glucosamine on tooth pulpal nociceptive responses in the rat
Effects of glucosamine on tooth pulpal nociceptive responses in the rat
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DOI：
10.1016/j.jds.2012.09.022
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发表时间：
2013-03-01
影响因子：
3.5
通讯作者：
Hayashi, Yoshihiko
中科院分区：
文献类型：
--
作者：
Kaida, Kei;Yamashita, Hiromi;Hayashi, Yoshihiko
背景/目的：D-氨基葡萄糖盐酸盐（DGL）具有多种生物活性，被认为是一种营养补充剂，可有效改善和治疗各种疾病，如骨关节炎和动脉粥样硬化。虽然，据报道，DGL在治疗骨关节炎中具有显著的疼痛缓解作用，但对其对牙痛的影响知之甚少。DGL作为控制牙髓痛疼痛的药物的适用性尚未报道。在这项研究中，使用在体外大鼠下颌下牙槽神经准备（颌神经准备），DGL对伤害性反应的影响在牙髓nerve.Materials和方法：DGL对伤害性反应的影响，为20只雄性Wistar白化病大鼠进行了评估，使用在体外颌神经准备。缓激肽（BK），作为一种化学伤害性兴奋剂，被应用于暴露的牙髓附近。结果：生理盐水组神经放电频率为2.06 ± 0.21 Hz（n = 10），DGL组为0.76 ± 0.16 Hz（n = 10）。结论：直接应用DGL可明显抑制BK诱发的伤害性反应。我们的研究结果表明，DGL可能有缓解牙痛的效果。中华民国口腔医学会版权所有（C）2012出版社：Elsevier Taiwan LLC All rights reserved.
Background/purpose: D-Glucosamine hydrochloride (DGL) has a variety of biological activities and is noted as a nutritional supplement that is effective for improvement and care of various disorders, such as osteoarthritis and atherosclerosis. Although, it has been reported that DGL has a significant pain relief effect in treating osteoarthritis, little is known about its effect on dental pain. The applicability of DGL as a medicament to control pain in pulpalgia has not been reported. In this study, using an in vitro rat mandible-inferior alveolar nerve preparation (jaw-nerve preparation), the effect of DGL on nociceptive responses in the tooth pulpal nerve was examined.Materials and methods: The effect of DGL on nociceptive responses for 20 male Wistar albino rats was evaluated using an in vitro jaw-nerve preparation. Bradykinin (BK), used as a chemical nociceptive stimulant, was applied near the exposed tooth pulp. Sixty seconds after BK application, the surface of the exposed pulp was treated with DGL solution or physiological saline (control).Results: The nerve firing rate was 2.06 +/- 0.21 Hz (n = 10) after 5 minutes of saline application, and 0.76 +/- 0.16 Hz (n = 10) after 5 minutes of DGL application. The DGL group showed significantly lower nerve firing rate than the control group.Conclusion: BK-induced nociceptive responses were significantly suppressed by direct application of DGL. Our results suggest that DGL might have a pain relief effect in dental pain. Copyright (C) 2012, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved.