Development of cyanine probes with dinitrobenzene quencher for rapid fluorogenic protein labelling
Development of cyanine probes with dinitrobenzene quencher for rapid fluorogenic protein labelling
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DOI：
10.1098/rsta.2017.0018
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发表时间：
2017-11-28
期刊：
影响因子：
5
通讯作者：
Kikuchi, Kazuya
中科院分区：
文献类型：
--
作者：
Hori, Yuichiro;Hirayama, Shinya;Kikuchi, Kazuya
使用蛋白质标签和荧光探针的多色蛋白质标记技术是活细胞中蛋白质时空分析的有力方法。由于花青荧光团具有吸引人的性质，多色成像的蛋白质，有一个巨大的需求，开发荧光花青探针在活细胞中的特异性蛋白质标记。在此，我们开发了荧光花青探针，用于通过使用二硝基苯荧光猝灭剂标记蛋白质标签。探针在标记反应后增强荧光强度，并发射橙子或远红色荧光。花青荧光团和二硝基苯猝灭剂之间的分子内相互作用不仅导致在自由状态下的探针的荧光猝灭，而且还促进标记反应。此外，探针成功地成像细胞表面蛋白质没有洗涤过程。这些发现提供了有价值的信息荧光花菁探针的设计，并表明，探针是有用的新的活细胞imaging tools.This文章的主题问题的一部分“化学在分子成像的挑战”。
A multicolour protein labelling technique using a protein tag and fluorogenic probes is a powerful approach for spatio-temporal analyses of proteins in living cells. Since cyanine fluorophores have attractive properties for multicolour imaging of proteins, there is a huge demand to develop fluorogenic cyanine probes for specific protein labelling in living cells. Herein, we develop fluorogenic cyanine probes for labelling a protein tag by using a dinitrobenzene fluorescence quencher. The probes enhanced fluorescence intensity upon labelling reactions and emitted orange or far-red fluorescence. Intramolecular interactions between the cyanine fluorophores and the dinitrobenzene quencher led not only to fluorescence quenching of the probes in the free state but also to promotion of labelling reactions. Furthermore, the probes successfully imaged cell-surface proteins without a washing process. These findings offer valuable information on the design of fluorogenic cyanine probes and indicate that the probes are useful as novel live-cell imaging tools.This article is part of the themed issue 'Challenges for chemistry in molecular imaging'.