Left ventricular functional assessment in mice: Feasibility of high spatial and temporal resolution ECG-gated blood pool SPECT
Left ventricular functional assessment in mice: Feasibility of high spatial and temporal resolution ECG-gated blood pool SPECT
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DOI：
10.1148/radiol.2452061973
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发表时间：
2007-11-01
期刊：
影响因子：
19.7
通讯作者：
Jaszczak, Ronald J.
中科院分区：
文献类型：
--
作者：
Chin, Bennett B.;Metzler, Scott D.;Jaszczak, Ronald J.
目的：探讨用心电图门控血池单光子发射计算机断层扫描（SPECT）评价小鼠左心室功能的可行性。材料和方法：所有的动物研究都得到了机构动物护理和使用委员会的批准。对正常小鼠（A组，n = 6）和心肌梗死小鼠（MI） （n = 8）进行常规时间帧采集（每ECG周期8帧）SPECT。为了确定高时间分辨率和快速数据采集的可行性，另一组正常小鼠（正常组B， n = 4）接受常规（八帧）时序和列表模式（LM）成像。以每ECG周期8帧和16帧以及10分钟的数据（短LM）重建LM采集。目视评估SPECT图像，并计算左肺背景活动比。用期相分析和阈值法确定左室收缩末期和舒张末期容积。采用左室容积法和计数法计算左室射血分数（LVEF） （n = 18只小鼠）。超声心动图分数缩短（FS）定义心肌梗死功能障碍（轻度心肌梗死：FS >= 50%；重度心肌梗死：FS < 50%）。采用方差分析比较组均值是否有显著差异。结果：心电图门控血池SPECT显示10只正常小鼠左室收缩正常。左肺背景比大于10:1（范围：10.3 - 29.4;n = 18）。在所有严重心肌梗死小鼠（n = 5）中，在SPECT上观察到局灶性壁运动异常，并进行相分析。重度心肌梗死小鼠LVEF明显低于正常A组小鼠(32% +/- 14[标准差]vs 64% +/- 8%
Purpose:To prospectively determine feasibility of evaluating murine left ventricular ( LV) function with electrocardiographically ( ECG)- gated blood pool single photon emission computed tomography ( SPECT).Materials and Methods: All animal studies had institutional animal care and use committee approval. SPECT was performed with conventional time- binned acquisition ( eight frames per ECG cycle) in normal mice ( normal group A, n = 6) and mice with myocardial infarction ( MI) ( n = 8). To determine feasibility of high temporal resolution and rapid data acquisition, another group of normal mice ( normal group B, n = 4) underwent imaging with conventional ( eight- frame) timebinned and list- mode ( LM) acquisitions. LM acquisitions were reconstructed with eight and 16 frames per ECG cycle and 10 minutes of data ( short LM). SPECT images were assessed visually, and LV- to - lung background activity ratios were calculated. LV end- systolic and end- diastolic volumes were defined with a phase analysis and threshold method. LV ejection fraction ( LVEF) was calculated from LV volumes and count- based methods ( n = 18 mice). Fractional shortening ( FS) at echocardiography defined MI dysfunction ( mild MI: FS >= 50%; severe MI: FS < 50%). Group means were compared for significant differences with analysis of variance.Results: ECG- gated blood pool SPECT demonstrated normal, concentric LV contraction in all normal mice ( n = 10). LV- to - lung background ratio was more than 10: 1 ( range, 10.3 - 29.4; n = 18). Focal wall motion abnormalities were detected at SPECT both visually and with phase analysis in all mice with severe MI ( n = 5). Mice with severe MI had significantly lower LVEF than normal group A mice ( 32% +/- 14 [ standard deviation] vs 64% +/- 8%; P