Compressive light-field microscopy for 3D neural activity recording
Compressive light-field microscopy for 3D neural activity recording
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DOI：
10.1364/optica.3.000517
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发表时间：
2016-05-20
期刊：
影响因子：
10.4
通讯作者：
Waller, Laura
中科院分区：
文献类型：
--
作者：
Pegard, Nicolas C.;Liu, Hsiou-Yuan;Waller, Laura
理解感知、认知和行为的机制需要能够同时高速记录和控制许多神经元电活动的仪器。全光学方法特别有前途，因为它们是微创的，并且可能可扩展到询问数千或数百万神经元的实验。传统的光场显微镜提供了一种具有良好的光效率和快速的单次拍摄3D荧光捕获方法，但由于在脑组织的深层中的散射而遭受低空间分辨率和显著的图像退化。在这里，我们提出了一种新的压缩光场显微镜方法来解决这两个问题，提供了一条测量大量组织中单个神经元活动的途径。该技术依赖于荧光信号的空间和时间稀疏性，允许人们在3D体积中识别和定位每个神经元，自然包括散射和畸变效应，而无需重建体积图像。对活斑马鱼的实验结果以100 Hz的采样率跟踪估计800多个神经结构的活动。(C)2016美国光学学会
Understanding the mechanisms of perception, cognition, and behavior requires instruments that are capable of recording and controlling the electrical activity of many neurons simultaneously and at high speeds. All-optical approaches are particularly promising since they are minimally invasive and potentially scalable to experiments interrogating thousands or millions of neurons. Conventional light-field microscopy provides a single-shot 3D fluorescence capture method with good light efficiency and fast speed, but suffers from low spatial resolution and significant image degradation due to scattering in deep layers of brain tissue. Here, we propose a new compressive light-field microscopy method to address both problems, offering a path toward measurement of individual neuron activity across large volumes of tissue. The technique relies on spatial and temporal sparsity of fluorescence signals, allowing one to identify and localize each neuron in a 3D volume, with scattering and aberration effects naturally included and without ever reconstructing a volume image. Experimental results on live zebrafish track the activity of an estimated 800+ neural structures at 100 Hz sampling rate. (C) 2016 Optical Society of America